LOCUS       sequence-163671-        9486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 
            selection. GFP was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and synthetic introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1137
FEATURES             Location/Qualifiers
     source          1..9486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    3568..3588
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3589..4188
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    4214..4238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4241..4261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5177..5248
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     protein_bind    complement(5250..5274)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      5279..5602
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(5606..5626)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5634..5651)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5634..5650)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7352..7371)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7471..7493
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7531..7549)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7617..7721
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7722..7790,7791..8582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7940..7959)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8753..9341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9242..9261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggcaggaaca
     3541 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttggc acctttggtc
     3601 ttttattgtc aacttccatt ggttcttcca ttgtttctgt taaattaatg aatttttcat
     3661 aaaataaaga cattatacaa tataaaaatg aagaatttat tgaaaataaa ctgccagaga
     3721 gaaaaagtat gcaacactcc cgccgagagt gtttgaaatg gtgtacggta cattttcgtg
     3781 ctaggagtta gatgtgcagg cagcaacgag agggggagag atttttttgg gccttgtgaa
     3841 attaacgtga gttttctggt catctgacta atcatgttgg ttttttgttg gtttattttg
     3901 tttttatctt tgtttttatc cagattagga aatttaaatt ttatgaattt ataatgaggt
     3961 caaacattca gtcccagcgt ttttcctgtt ctcactgttt agtcgaattt ttattttagg
     4021 ctttcaacaa atgttctaac tgtcttattt gtgacctcac tttttatatt tttttaattt
     4081 ttaaaaatat tagaagtttc taggataatt ttttcgactt ttattctctc taccgtccgc
     4141 actcttctta cttttaaatt aaattgtttt tttttcagtt gggaaacact ttgctcactc
     4201 cgtagcagcc atggcaagtt tgtacaaaaa agcaggctcg ccaaagaaga agcgtaaggt
     4261 gtccaaggta agttttctag agacctttac taactaacta ggtctcatag attttcaggg
     4321 agaggagctc ttcaccggag tcgtcccaat cctcgtcgag ctcgacggag acgtcaacgg
     4381 acacaagttc tccgtctccg gagagggaga gggagacgcc acctacggaa agctcaccct
     4441 caagttcatc tgcaccaccg gaaagctccc agtcccatgg ccaaccctcg tcaccacctt
     4501 ctgctacgga gtccaatgct tctcccgtta cccagaccac atgaagcgtc acgacttctt
     4561 caagtccgcc atgccagagg gatacgtcca agagcgtacc atcttcttcg taagttatgt
     4621 agagaccttt actaactaac taggtctcaa acattttcag aaggacgacg gaaactacaa
     4681 gacccgtgcc gaggtcaagt tcgagggaga caccctcgtc aaccgtatcg agctcaaggg
     4741 aatcgacttc aaggaggacg gaaacatcct cggacacaag ctcgagtaca actacaactc
     4801 ccacaacgtc tacatcatgg ccgacaagca aaagaacgga atcaaggtca acttcaaggt
     4861 aagttcttta gagaccttta ctaactaact aggtctcagt aattttcaga tccgtcacaa
     4921 catcgaggac ggatccgtcc aactcgccga ccactaccaa caaaacaccc caatcggaga
     4981 cggaccagtc ctcctcccag acaaccacta cctctccacc caatccgccc tctccaaggt
     5041 aagttcataa gagaccttta ctaactaact aggtctcaga atttttcagg acccaaacga
     5101 gaagcgtgac cacatggtcc tcctcgagtt cgtcaccgcc gccggaatca cccacggaat
     5161 ggacgagctc tacaagtccc gtcgtcgtaa ggccaaccca accaagctct ccgagaacgc
     5221 caagaagctc gccaaggagg tcgagaacaa cccagctttc ttgtacaaag tgggataaat
     5281 gcaaaatcct ttcaagcatt cccttcttct ctatcactct tctttctttt tgtcaaaaaa
     5341 ttctctcgct aatttatttg cttttttaat gttattattt tatgactttt tatagtcact
     5401 gaaaagtttg catctgagtg aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt
     5461 tcacaatctc tcttcaattc cattttgaag tgctttaaac ccgaaaggtt gagaaaaatg
     5521 cgagcgctca aatatttgta ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta
     5581 ttgtgccgcc aagaaaaaag tctcacaact ttattataca tagttgataa ttcactggcc
     5641 gtcgttttac actcgagacg tacggtgcgc gcgatgcatt cgaagatctg cccactagtg
     5701 agtcgtatta taagtgcaag taagatcagt gtttgtttcg atatcagtct gtttcgtaac
     5761 ggtcttctgt ataactacaa aaaagaataa aaaaccgtat cacaactgag aaaaaagaca
     5821 aaacttattg aacagtgttc ctttcaaaaa attattttgt aaaaaatcga gtttttctcc
     5881 aaaataacgc acgcaatcaa aaacaagaaa actaacgtgt tctcctgggc gtggaactcc
     5941 ttatcaagat taccattatt gtctgtcaga attgatattt caattatttc atatctagac
     6001 aaacccgggt gtcttgcatt tattagtact gtgagtttac atacaaacga ggcttactca
     6061 gataacatag agtcacctct aattagcaaa aacccatttc taattagcta tttcgaccag
     6121 ttctaaagtc accgtaatta gaaatggttt ttgtagcttt tctaattaga aatgaccccg
     6181 agataagtga aatatggcat tttctgggtt ttctagtaag aaatgatccc gcacgtagtg
     6241 attccgcgtt acaagccaaa ttcaaattaa aaaagtatat tacacaattc aagttacatt
     6301 attttcagga aacaatggcg aagagcaaag cagagattca aaaggaggaa agagacaaca
     6361 gatatgaacg aagacttctt gaaaagagaa agagacatga ggtgagtaac catgataata
     6421 aataatcaag taagggctat agatttataa attctggagt taacagtttc gaaaaaaagt
     6481 agttttcata tgtgcgaagg aatatcaaaa ataggggaat agcctaaacg agtgagaaaa
     6541 ttaaatgttt gttatttttt caaaaatgtc tgcagggccg ttatcctttc aaatgcactc
     6601 tatcaaacat agatcatatc catcatatct tatccagaat ttatatcata tccaagattg
     6661 agagcgtttc aaggaaaatc tcaaacaacc atacaatttt gtagatcgac aacaaggcct
     6721 ccacttcttc atcgatggcc tctgcagcac ctgcaccacc aagtttgtat ttttcatatt
     6781 aatataaagt gagccacgca tagtcgtttt taggattttt gccagatcga tgtcgcgcaa
     6841 acagactgtt tttttttctt tgaaagccgt gcgtgacatg ggccacggat tgtaacgccg
     6901 cgctggatgg ataaaaattt attcagtttt ttagccttta caaaatatgg ttcgactact
     6961 ttctttattt ttacattttt ataggtgctc attgagctga gatattttgg gtataatcgg
     7021 aaaatcgctg attttggtca tttttctgca ctaaagatta cacaaaaagt tcagtattta
     7081 gattcattgt tgaaaattgg ggatgatcta cagatacata tcgccgtgtt caaaaacaca
     7141 cgtgacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7201 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7261 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     7321 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     7381 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     7441 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     7501 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     7561 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     7621 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7681 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7741 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7801 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7861 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7921 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7981 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8041 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8101 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8161 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8221 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8281 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8341 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8401 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8461 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8521 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8581 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8641 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8701 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8761 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8821 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8881 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8941 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9001 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9061 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9121 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9181 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9241 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9301 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9361 ttttacggtt cctggtcttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9421 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccagc ggataacaat
     9481 ttcaca
//
