LOCUS       sequence-163680-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pJB38 plasmid containing the S.aureus Newman genetic region between 
            genes 29 and 30.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pJB38-NWMN2930
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3811..3829
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    3900..3918
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             3940..4566
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4016..4035)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(6557..6579)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6665..6805
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6720..6739
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6820..6837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6991..7579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7071..7090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7750..8541,8542..8610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8373..8392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8611..8715)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8783..8801
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattcagtg gctacattcg aacatatcaa agcatacgat agaaaaacaa taagttatga
       61 tatgttacat gtaacaccac ctatgggtcc cttagatgta gtaaaagaaa gtacactttc
      121 agatagtgag ggttgggtag atgttaaccc aaccacatta cagcataaaa gctactctaa
      181 tgtatttgca cttggtgatg cttcaaatgt acctacttca aaaacaggcg cagctattcg
      241 taagcaagca cctatcgtcg ctaataattt attgcaagtg atgaataatc aaatgttaac
      301 gcatcattat gatggttata cttcatgccc tattgttact ggatataata ggttaatact
      361 tgcagagttt gattataata aaaatactaa agaaacaatg ccgtttaatc aggccaaaga
      421 acgtagaagt atgtatatat ttaagaaaga tttattacct aaaatgtatt ggtacggcat
      481 gctaaaagga ttaatataat aaagtacaga aaacaataaa tttttaatga aaaatctttt
      541 actataaaag attaagtatt taaatgacgt gtcagtgttg tgtttatatg tcgtgaattt
      601 ttagctctaa atagtataag attgaaaaag ttgttactgt tttaaatgat cacgatgaag
      661 tcattcaata agaatgatta tgaaaataga aacagcagta agatattttc taattgaaaa
      721 tcatctcact gctgtttttt aaaggtttat acctcatcct ctaaattatt taaaaataat
      781 taatggtatt tgagcacgtt tagcgacttt atgactgaca ttaccaattt ccatttcttg
      841 ccagatattc aaaccacgtg tactcaaaat gatagcttgg tatgtacctc caatagtaat
      901 ttcaataact ttgtctgttg aacactaaga gcaattttaa tttcataatg tgttgtaaac
      961 attttttttg attggagttt ttttctgagt taaacgatat cctgatgtat ttttaatttt
     1021 gcaccatttc caaaaggata agtgacataa gtaaaaaggc atcatcggga gttatcctat
     1081 caggaaaacc aagataatac ctaagtagaa agtgttcaat ccgtgttaaa ttgggaaata
     1141 tcatccataa actttattac tcatactata attcaatttt aacgtcttcg tccatttggg
     1201 cttcaaattc atcgagtagt gctcgtgctt ctgcaattga ttgtgtgttc atcaattgat
     1261 gtcgaagttc gctagcgcct cttatgccac gcacatagat tttaaagaat ctacgcaagc
     1321 tcttgaattg tcgtatttca tctttttcat atttgttaaa caatgataaa tgcaatctca
     1381 atagatctaa tagttccttg cttgtgtgtt cgcgtggttc tttttcaaaa gcgaatggat
     1441 tgtggaaaat gcctctacca atcatgacgc catcaatgcc atatttttct gccagttcaa
     1501 gtcctgtttt tctatcggga atatcaccgt taattgttaa caatgtattt ggtgcaattt
     1561 cgtcacgtaa atttttaata gcttcgatta attcccaatg tgcatctact ttactcattt
     1621 ctttacgtgt acgaagatga atagataaat tggcaatgtc ttgttcgaag acgtgcttca
     1681 accaatcttt ccattcatcg atttcatagt agccaaggcg tgttttaaca cttaccggaa
     1741 gcccacctgc tttagtcgct tgaataattt cggcagcaac gtcaggtctt aagattaagc
     1801 cggaaccctt acggtacccg gggatcctct agagtcgacc tgcaggcatg caagctctga
     1861 tttcactttt tgcattctac aaactgcata actcatatgt aaatcgctcc tttttaggtg
     1921 gcacaaatgt gaggcatttt cgctctttcc ggcgaggcta gttaccctta agttattggt
     1981 atgactggtt ttaagcgcaa aaaaagttgc tttttcgtac ctattaatgt atcgttagaa
     2041 aaccgactgt aaaaagtaca gtcggcatta tctcatatta taaaagccag tcattaggcc
     2101 tatctgacaa ttcctgaata gagttcataa acaatcctgc atgataacca tcacaaacag
     2161 aatgatgtac ctgtaaagat agcggtaaat atattgaatt acctttatta atgaattttc
     2221 ctgctgtaat aatgggtaga aggtaattac tattattatt gatatttaag ttaaacccag
     2281 taaatgaagt ccatggaata atagaaagag aaaaagcatt ttcaggtata ggtgttttgg
     2341 gaaacaattt ccccgaacca ttatatttct ctacatcaga aaggtataaa tcataaaact
     2401 ctttgaagtc attctttaca ggagtccaaa taccagagaa tgttttagat acaccatcaa
     2461 aaattgtata aagtggctct aacttatccc aataacctaa ctctccgtcg ctattgtaac
     2521 cagttctaaa agctgtattt gagtttatca cccttgtcac taagaaaata aatgcagggt
     2581 aaaatttata tccttcttgt tttatgtttc ggtataaaac actaatatca atttctgtgg
     2641 ttatactaaa agtcgtttgt tggttcaaat aatgattaaa tatctctttt ctcttccaat
     2701 tgtctaaatc aattttatta aagttcattt gatatgcctc ctaaattttt atctaaagtg
     2761 aatttaggag gcttacttgt ctgctttctt cattagaatc aatccttttt taaaagtcaa
     2821 tattactgta acataaatat atattttaaa aatatcccac tttatccaat tttcgtttgt
     2881 tgaactaatg ggtgctttag ttgaagaata aagaccacat taaaaaatgt ggtcttttgt
     2941 gtttttttaa aggatttgag cgtagcgaaa aatccttttc tttcttatct tgataataag
     3001 ggtaactatt gccgggatag actgtaacat tctcacgcat aaaatcccct ttcattttct
     3061 aatgtaaatc tattacctta ttattaattc aattcgctca taattaatcc tttttcttat
     3121 tacgcaaaat ggcccgattt aagcacaccc tttattccgt taatgcgcca tgacagccat
     3181 gataattact aatactagga gaagttaata aatacgggtg atctaatgat tcaatgattc
     3241 aaacccttgt gaacttcttt agaacaaaag aggttcgtaa caagattttc ttcacactag
     3301 caatgttagt aatttttaaa atagggactt atataccagc tccaggagta aatcctgcag
     3361 cttttgataa tccccaaggt tctcaaggtg ccactgagtt attaaatact tttggtggcg
     3421 gagccttgaa acgattttct atttttgcaa tgggtattgt accctacatc actgcatcaa
     3481 tcgtaatgca attattacaa atggatattg tccctaaatt ctcagaatgg gcaaaacaag
     3541 gtgaagtagg tagaagaaag ttaaataacg ttactcgtta tttagcaatt tctttagcat
     3601 ttatccaatc tataggtatg gcattccaat ttaataatta tctcaaaggt gcgctgatta
     3661 tcaatcagtc aattatgagt tatttattaa tagcactagt tttgacagca ggaactgctt
     3721 tcttaatatg gcttggtgat caaatcactc agttcggtgt tggtaatggt atttctatta
     3781 tcatattccc atcaagctta ttttaattat actctatcaa tgatagagtg tcaatatttt
     3841 ttttagtttt tcatgaactc gatcgagggg atccaaataa aaaactagtt tgacaaataa
     3901 ctctatcaat gatagagtgt caacaaaaag gaggaattaa tgatgtctag attagataaa
     3961 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
     4021 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
     4081 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac
     4141 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
     4201 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
     4261 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     4321 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg
     4381 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     4441 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
     4501 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
     4561 tcttaaaagc agcataacct ttttccgtga tggtaacttc acggtaacca agataacaaa
     4621 gaatacaaga aaatatttac aaaaaatcaa tttaacaatt ccttaaaaca tgcaggaatt
     4681 gacgatttaa acaatattag ctttgaacaa ttcttatctc ttttcaatag ctataaatta
     4741 tttaataagt aagttaaggg atgcataaac tgcatccctt aacttgtttt tcgtgtgcct
     4801 attttttgtg aatcgattat gtcttttgcg cagtcggctt aaaccagttt tcgctggtgc
     4861 gaaaaaagag tgtcttgtga cacctaaatt caaaatctat cggtcagatt tataccgatt
     4921 tgattttata tattcttgaa taacatacgc cgagttatca cataaaagcg ggaaccaatc
     4981 atcaaattta aacttcattg cataatccat taaactctta aattctacga ttccttgttc
     5041 atcaataaac tcaatcattt ctttaattaa tttatatcta tctgttgttg ttttctttaa
     5101 taattcatca acatctacac cgccataaac tatcatatct tctttttgat atttaaattt
     5161 attaggatcg tccatgtgaa gcatatatct cacaagacct ttcacacttc ctgcaatctg
     5221 cggaatagtc gcattcaatt cttctgtaat tatttttatc tgttcataag atttattacc
     5281 ctcatacatc actagaatat gataatgctc ttttttcatc ctatcttctg tatcagtatc
     5341 cctatcatgt aatggagaca ctacaaattg aatgtgtaac tcttttaaat actctaacca
     5401 ctcggctttt gctgattctg gatataaaac aaatgtccaa ttacgtcctc ttgaattttt
     5461 cttgttttca gtttctttta ttacattttc gctcatgata taataacggt gctaatacat
     5521 ttaacaaaat ttagtcatag ataggcagca tgccagtgct gtctatcttt ttttgtttaa
     5581 aatgcaccgt attcctcctt tgcatatttt tttattagaa taccggttgc atctgatttg
     5641 ctaatattat atttttcttt gattctattt aatatctcat tttcttctgt tgtaagtctt
     5701 aaagtaacag caactttttt ctcttctttt ctatctacaa ccatcactgt acctcccaac
     5761 atctgttttt ttcactttaa cataaaaaac aaccttttaa cattaaaaac ccaatattta
     5821 tttatttgtt tggacaatgg acaatggaca cctagggggg aggtcgtagt acccccctat
     5881 gttttctccc ctaaataacc ccaaaaatct aagaaaaaaa gacctcaaaa aggtctttaa
     5941 ttaacatctc aaatttcgca tttattccaa tttccttttt gcgtgtgatg cgctgcgtcc
     6001 attaaaaatc ctagagcttt gcaaccgaaa gttaatagct gtcgctacta ctttcgctta
     6061 cgctctaagt atattttaag gactgtcaca cgcaaaaagt tttctcggca taaaagtacc
     6121 tctacatctc taaatcgtct gtacgctgtt tctcacgctt tctatcgacc ttctggacat
     6181 tatcctgtac aacatccata aactgtccca cacgctcgaa tttggaatca ttaaagaatt
     6241 tctctttaag cctattaaac cctttctcaa acccagggaa attcgccctc gcagcacgat
     6301 ataaagtcac tgtactatct tgaaatttct ctgatacatt caactgctca ttcaaactat
     6361 cattctctcg ctttaattta ttaacctctt tacttttttc gtgatacccc tctttccatg
     6421 tattcactac ttctttcaaa ctctctctac gtttttttaa ttcttgattt tctgtgtaat
     6481 agtctgtgct cttaatattt tcgtaatcat caacaatccg ttcctgcctc gcgcgtttcg
     6541 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     6601 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     6661 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     6721 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     6781 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6841 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6901 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6961 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7021 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7081 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7141 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7201 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7261 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7321 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7381 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     7441 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7501 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7561 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7621 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7681 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7741 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7801 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7861 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7921 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7981 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8041 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     8101 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8161 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8221 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8281 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8341 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     8401 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8461 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8521 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8581 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8641 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8701 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     8761 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaa
//
