LOCUS       sequence-163684-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: MAP3K7CL_pCSdest
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..36
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(466..490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    534..668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(558..577)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     612..631
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(781..801)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(781..799)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(820..836)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(820..836)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(833..855)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    844..860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attcaaggat cacaagtttg tacaaaaaag caggcaccat gatcagcaca gccagggtac
       61 ctgctgacaa gcctgtacgc atcgccttta gcctcaatga cgcctcagat gatacacccc
      121 ctgaagactc cattcctttg gtctttccag aattagacca gcagctacag cccctgccgc
      181 cttgtcatga ctccgaggaa tccatggagg tgttcagaca gcactgccaa atagcagaag
      241 aataccttga ggtcaaaaag gaaatcaccc tgcttgagca aaggaagaag gagctcattg
      301 ccaagttaga tcaggcagaa gaggagaagg tggatgctgc tgagctggtt cgggaattcg
      361 aggctctgac ggaggagaat cggacgttga ggttggccca gtctcaatgt gtggaacaac
      421 tggagaaact tcgaatacag tatcagaaga ggcagggctc gtccaaccca gctttcttgt
      481 acaaagtggt gatcctctcg agcctctaga actatagtga gtcgtattac gtagatccag
      541 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      601 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      661 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      721 aggtttttta attcgcggcc gcggcgccaa tgcattgggc ccggtaccca gcttttgttc
      781 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      841 aaattgttat ccgctcacaa ttccacacaa catacgagcc gggagcataa
//
