LOCUS       sequence-163685-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: MAP3K7CL_pCSdest
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(492..516)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(570..588)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(571..588)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gttggacgag ccctgcctct tctgatactg tattcgaagt ttctccagtt gttccacaca
      121 ttgagactgg gccaacctca acgtccgatt ctcctccgtc agagcctcga attcccgaac
      181 cagctcagca gcatccacct tctcctcttc tgcctgatct aacttggcaa tgagctcctt
      241 cttcctttgc tcaagcaggg tgatttcctt tttgacctca aggtattctt ctgctatttg
      301 gcagtgctgt ctgaacacct ccatggattc ctcggagtca tgacaaggcg gcaggggctg
      361 tagctgctgg tctaattctg gaaagaccaa aggaatggag tcttcagggg gtgtatcatc
      421 tgaggcgtca ttgaggctaa aggcgatgcg tacaggcttg tcagcaggta ccctggctgt
      481 gctgatcatg gtgcctgctt ttttgtacaa acttgtgatc cttgaattcg aatcgatggg
      541 atcctgcaaa aagaacaagt agcttgtatt ctatagtgtc acctaaatca agcttgctcc
      601 gacgtcccca ggcagaatgg cggttcccta aacgagcatt gcttatatag acctcccatt
      661 aggcacgcct accgcccatt tacgtcaatg gaacgcccat ttgcgtcatt gcccctcccc
      721 attgacgtca atggggatgt acttggcagc catcgcgggc catttaccgc cattgacgtc
      781 aatgggagta ctgccaatgt accctggcgt acttccaata gtaatgtact tgccaagtta
      841 ctattaatag atattgatgt actgccaagt gg
//
