LOCUS       sequence-163689-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: BAGE4_pCSdest
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..36
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(221..245)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    289..423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(313..332)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     367..386
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(536..556)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(536..554)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(575..591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(575..591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(588..610)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    599..615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(623..629,630..647,648..653))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    668..689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(806..823)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attcaaggat cacaagtttg tacaaaaaag caggcaccgt acaaaaaagc agaagggccg
       61 tcaaggccca ccatggcagc cggagcggtt tttctggcat tgtctgccca gctgctccaa
      121 gccaggctga tgaaggagga gtcccctgtg gtgagctggt ggttggagcc tgaagacggc
      181 acagctctgt agggcctcat gggcccagct ttcttgtaca acccagcttt cttgtacaaa
      241 gtggtgatcc tctcgagcct ctagaactat agtgagtcgt attacgtaga tccagacatg
      301 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      361 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      421 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
      481 ttttaattcg cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt
      541 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      601 gttatccgct cacaattcca cacaacatac gagccgggag cataaagtgt aaagcctggg
      661 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      721 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      841 tgcggcgagc ggtatcagct cactcaa
//
