LOCUS       sequence-163690-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: BAGE4_pCSdest
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(247..271)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(325..343)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(326..343)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gttgtacaag aaagctgggc ccatgaggcc ctacagagct gtgccgtctt caggctccaa
      121 ccaccagctc accacagggg actcctcctt catcagcctg gcttggagca gctgggcaga
      181 caatgccaga aaaaccgctc cggctgccat ggtgggcctt gacggccctt ctgctttttt
      241 gtacggtgcc tgcttttttg tacaaacttg tgatccttga attcgaatcg atgggatcct
      301 gcaaaaagaa caagtagctt gtattctata gtgtcaccta aatcaagctt gctccgacgt
      361 ccccaggcag aatggcggtt ccctaaacga gcattgctta tatagacctc ccattaggca
      421 cgcctaccgc ccatttacgt caatggaacg cccatttgcg tcattgcccc tccccattga
      481 cgtcaatggg gatgtacttg gcagccatcg cgggccattt accgccattg acgtcaatgg
      541 gagtactgcc aatgtaccct ggcgtacttc caatagtaat gtacttgcca agttactatt
      601 aatagatatt gatgtactgc caagtgggcc atttaccgtc attgacgtca atagggggcg
      661 tgagaacgga tatgaatggg caatgagcca tcccattgac gtcaatggtg ggtggtccta
      721 ttgacgtcaa tgggcattga gccaggcggg ccatttaccg taattgacgt caatggggga
      781 ggcgccatat acgtcaatag gaccgcccat atgacgtcaa taggaaagac catatataga
      841 gaccattgac gtcaatgggg gagtggctat gggcggtatt aggaagcc
//
