LOCUS       sequence-163712-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FnR.0.6.2-Y78T protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Johnson SM, Javner C, Hackel BJ
  TITLE     Development and Implementation of a Protein–Protein Binding 
            Experiment To Teach Intermolecular Interactions in High School or 
            Undergraduate Classrooms
  JOURNAL   J. Chem. Educ. 2017, 94, 3, 367–374
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Chem. 
            Educ. 2017, 94, 3, 367–374"
COMMENT     Sequence Label: pET - FnR062.Y78T
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             365..387
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    402..426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(426..445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(427..445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(529..548)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        754..831
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(851..870)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tttgttagca gccggatctc gtttagttaa tcagtgatga tggtgatggt gggatccctg
       61 agacggtttg tcgatttcgg tgcgataatt gatgctgatt gggtttgaat cgtcgttagt
      121 accggttaca gcgtacacgg taatggtata atcctggccc ggtttcagac cgctgatggt
      181 cgcattagaa taagatctcg gaacagtgaa ttcctggctc ggggagttac caccagtttc
      241 gccgtaggtg atacggtaat agatagcgtg ataaggatcg tcccaagaaa tagtcagaga
      301 gttcggagtt gcgttggtaa cctccaggtt acgcggagag tcggaggagc tagccatatg
      361 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      421 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
      481 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      541 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      601 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
      661 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
      721 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt
      781 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
      841 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
      901 gtgaaccagg ccagccacgt ttctgcgaaa acgcggga
//
