LOCUS       sequence-163721-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FnR.0.6.2-Y78W protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Johnson SM, Javner C, Hackel BJ
  TITLE     Development and Implementation of a Protein–Protein Binding 
            Experiment To Teach Intermolecular Interactions in High School or 
            Undergraduate Classrooms
  JOURNAL   J. Chem. Educ. 2017, 94, 3, 367–374
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Chem. 
            Educ. 2017, 94, 3, 367–374"
COMMENT     Sequence Label: pET - FnR062.Y78W
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             338..355
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(420..438)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      434..481
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      518..973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(605..624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     815..836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatggctagc tcctccgact ctccgcgtaa
       61 cctggaggtt accaacgcaa ctccgaactc tctgactatt tcttgggacg atccttatca
      121 cgctatctat taccgtatca cctacggcga aactggtggt aactccccga gccaggaatt
      181 cactgttccg agatcttatt ctaatgcgac catcagcggt ctgaaaccgg gccaggatta
      241 taccattacc gtgtacgctg taaccggttg gaacgacgat tcaaacccaa tcagcatcaa
      301 ttatcgcacc gaaatcgaca aaccgtctca gggatcccac catcaccatc atcactgatt
      361 aactaaacga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      421 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      481 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      721 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      841 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
      901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
      961 acgcttacaa tttaggtggc acttttcggg gaaatgtgc
//
