LOCUS       sequence-163735-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-myc-Rac1-Q61L
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..68
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(78..254,255..257,258..653)
                     /codon_start=1
                     /product="constitutively active mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (Q61L)
                     /translation="QAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGLEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    686..820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(723..742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     777..796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 actgcacctc ggttctatcg attgaattgg ccaccatgga gcagaagctg atctccgagg
       61 aggacctggg atccccgcag gccatcaagt gtgtggtggt gggagacgga gctgtaggta
      121 aaacttgcct actgatcagt tacacaacca atgcatttcc tggagaatat atccctactg
      181 tctttgacaa ttattctgcc aatgttatgg tagatggaaa accggtgaat ctgggcttat
      241 gggatacagc tggactagaa gattatgaca gattacgccc cctatcctat ccgcaaacag
      301 atgtgttctt aatttgcttt tcccttgtga gtcctgcatc atttgaaaat gtccgtgcaa
      361 agtggtatcc tgaggtgcgg caccactgtc ccaacactcc catcatccta gtgggaacta
      421 aacttgatct tagggatgat aaagacacga tcgagaaact gaaggagaag aagctgactc
      481 ccatcaccta tccgcagggt ctagccatgg ctaaggagat tggtgccgta aaatacctgg
      541 agtgctcggc gctcacacag cgaggcctca agacagtgtt tgacgaagcg atccgagcag
      601 tcctctgccc gcctcccgtg aagaagagga agagaaaatg cctgctgttg taagaattcc
      661 tgcagaagct tggccgccat ggcccaactt gtttattgca gcttataatg gttacaaata
      721 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      781 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gggaattaat tcggcgcagc
      841 accatggcct gaaataacct ctgaaagagg aacttgg
//
