LOCUS       sequence-163738-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging 
            (the recipient vector can be TetON 3G promoter system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2013-12-19- 
            19"; volume: "155"; issue: "7"; pages: "1479-91"
COMMENT     Sequence Label: pSLQ1658-dCas9-EGFP
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctcg ccaccatggc tagccccaaa
       61 aagaagagga aagtggacaa gaagtattct atcggactgg ccatcgggac taatagcgtc
      121 gggtgggccg tgatcactga cgagtacaag gtgccctcta agaagttcaa ggtgctcggg
      181 aacaccgacc ggcattccat caagaaaaat ctgatcggag ctctcctctt tgattcaggg
      241 gagaccgctg aagcaacccg cctcaagcgg actgctagac ggcggtacac caggaggaag
      301 aaccggattt gttaccttca agagatattc tccaacgaaa tggcaaaggt cgacgacagc
      361 ttcttccata ggctggaaga atcattcctc gtggaagagg ataagaagca tgaacggcat
      421 cccatcttcg gtaatatcgt cgacgaggtg gcctatcacg agaaataccc aaccatctac
      481 catcttcgca aaaagctggt ggactcaacc gacaaggcag acctccggct tatctacctg
      541 gccctggccc acatgatcaa gttcagaggc cacttcctga tcgagggcga cctcaatcct
      601 gacaatagcg atgtggataa actgttcatc cagctggtgc agacttacaa ccagctcttt
      661 gaagagaacc ccatcaatgc aagcggagtc gatgccaagg ccattctgtc agcccggctg
      721 tcaaagagcc gcagacttga gaatcttatc gctcagctgc cgggtgaaaa gaaaaatgga
      781 ctgttcggga acctgattgc tctttcactt gggctgactc ccaatttcaa gtctaatttc
      841 gacctggcag aggatgccaa gctgcaactg tccaaggaca cctatgatga cgatctcgac
      901 aacctcctgg cccagatcgg tgaccaatac gccgaccttt t
//
