LOCUS       sequence-163739-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose inducible lambda red and anhydrotetracycline inducible 
            sgRNA expression that targets pCas9 plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Reisch CR, Prather KL
  TITLE     The no-SCAR (Scarless Cas9 Assisted Recombineering) system for 
            genome editing in Escherichia coli.
  JOURNAL   Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.
  PUBMED    26463009
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-14"; volume: "5:15096. doi"; pages: " 
            10.1038/srep15096"
COMMENT     Sequence Label: pKDsgRNA-p15
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..73
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             82..498
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
ORIGIN
        1 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt gggaattcga
       61 gctctaagga ggttataaaa aatggatatt aatactgaaa ctgagatcaa gcaaaagcat
      121 tcactaaccc cctttcctgt tttcctaatc agcccggcat ttcgcgggcg atattttcac
      181 agctatttca ggagttcagc catgaacgct tattacattc aggatcgtct tgaggctcag
      241 agctgggcgc gtcactacca gcagctcgcc cgtgaagaga aagaggcaga actggcagac
      301 gacatggaaa aaggcctgcc ccagcacctg tttgaatcgc tatgcatcga tcatttgcaa
      361 cgccacgggg ccagcaaaaa atccattacc cgtgcgtttg atgacgatgt tgagtttcag
      421 gagcgcatgg cagaacacat ccggtacatg gttgaaacca ttgctcacca ccaggttgat
      481 attgattcag aggtataaaa cgaatgagta ctgcactcgc aacgctggct gggaagctgg
      541 ctgaacgtgt cggcatggat tctgtcgacc cacaggaact gatcaccact cttcgccaga
      601 cggcatttaa aggtgatgcc agcgatgcgc agttcatcgc attactgatc gttgccaacc
      661 agtacggcct taatccgtgg acgaaagaaa tttacgcctt tcctgataag cagaatggca
      721 tcgttccggt ggtgggcgtt gatggctggt cccgcatcat caatgaaaac cagcagtttg
      781 atggcatgga ctttgagcag gacaatgaat cctgtacatg ccggatttac cgcaaggacc
      841 gtaatcatcc gatctgcgtt accgaatgga tggatgaatg ccgccgcgaa ccattcaaaa
      901 ctcgcgaagg cagagaaatc acggggccgt ggcagtcgca tcccaa
//
