LOCUS       sequence-163744-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for cloning and expressing your gene of interest. 
            Hygromycin selection. This plasmid is not available at Addgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Morgenstern JP, Land H
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990-06-25"; volume: "18(12)"; pages: "3587-96"
COMMENT     Sequence Label: pBABE-hygro
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..80
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     96..112
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(186..206)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        191..520
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      371..506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
       61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      121 taggcgccgg ccggatccca gtgtggtggt acgtaggaat tcgccagcac agtggtcgac
      181 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
      241 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      301 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
      361 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      421 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
      481 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgcca gctggggcgc
      541 cctctggtaa ggttgggaag ccctgcagat ctgatcccgg ggggcaatga gatatgaaaa
      601 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
      661 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      721 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      781 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
      841 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
      901 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc g
//
