LOCUS       sequence-163745-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et 
            al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the 
            Arabidopsis U6 promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S
  TITLE     Targeted mutagenesis in the model plant Nicotiana benthamiana using 
            Cas9 RNA-guided endonuclease.
  JOURNAL   Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655.
  PUBMED    23929340
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08-8- 8"; volume: "31"; 
            issue: "8"; pages: "691-3"
COMMENT     Sequence Label: pICSL01009::AtU6p
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        309..383
                     /label=AtU6 promoter
                     /note="consensus promoter from the three Arabidopsis U6 
                     snRNA genes (Waibel and Filipowicz, 1990; Nekrasov et al., 
                     2013)"
     primer_bind     complement(420..438)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        506..610
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      181 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      241 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt
      301 ctcaggagtg atcaaaagtc ccacatcgat caggtgatat atagcagctt agtttatata
      361 atgatagagt cgacatagcg attgtgagac cacgaagtgg ctcttcagtg gacgaaaggg
      421 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      481 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      541 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      601 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
      661 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
      721 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
      781 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
      841 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
      901 ctcgaaccga cgttgctggc cgtacatttg tacggctc
//
