LOCUS       sequence-163751-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of human POLQ K121M mutant (a mutation in the 
            conserved ATP-binding site of the Walker A motif in the 
            helicase-like domain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, 
            Bylund GO, Doublie S, Johansson E, Ramsden DA, McBride KM, Wood RD
  TITLE     Mechanism of suppression of chromosomal instability by DNA 
            polymerase POLQ.
  JOURNAL   PLoS Genet. 2014 Oct 2;10(10):e1004654. doi: 
            10.1371/journal.pgen.1004654. eCollection 2014 Oct.
  PUBMED    25275444
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2014-10-2"; volume: "10(10):e1004654. doi"; pages: "
            10.1371/journal.pgen.1004654. eCollection 2014 Oct"
COMMENT     Sequence Label: pCDH-EF1-FHC-POLQ-K121M
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..100)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(74..100)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(116..139)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg ggagggagag
       61 gggcgcggcc ctaggcgtag tcgggcacgt cgtaggggta tcctccagcg gccgacttgt
      121 catcgtcgtc cttgtagtct cctccagcgg ccgccacatc aaagtccttt agctctcccc
      181 agctggcgcc tattttcact ttcactttca atttcacaga cagttttaca gcactttcca
      241 tttcattctt gacaatctga gctacctgaa caacatcttc ttctgccact tcatatagga
      301 gttcatcatg gagttgaagg atgaagaagc ctcctctgat tgggcagaac atcccttgca
      361 gttttctctt tcgtgacaat cctgttcggt cactttggag cataccctct cgatgaccat
      421 gggatttgaa ggttgagtgg aaggtctcta attgcttctg aatgttaact gtggctattt
      481 tgacaatatc agctgctgat ccttggacta ttgtgttgat agcttgacgc tcagcgtgag
      541 ctttacgata agggttgttg tctttgattc ctggcaaata tctacgcctt cccaaaatgg
      601 tctgaacaaa tccgtctctt ttacaattct tcactgt
//
