LOCUS       sequence-163753-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HA tagged ADAM10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Gschwind A, Hart S, Fischer OM, Ullrich A
  TITLE     TACE cleavage of proamphiregulin regulates GPCR-induced 
            proliferation and motility of cancer cells.
  JOURNAL   EMBO J. 2003 May 15;22(10):2411-21.
  PUBMED    12743035
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2003-05-15"; volume: "22(10)"; pages: "2411-21"
COMMENT     Sequence Label: pcDNA3-ADAM10-HA
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgccacca tggtgttgct gagagtgtta attctgctcc tctcctgggc
      121 ggcggggatg ggaggtcagt atgggaatcc tttaaataaa tatatcagac attatgaagg
      181 attatcttac aatgtggatt cattacacca aaaacaccag cgtgccaaaa gagcagtctc
      241 acatgaagac caattttcac gtctagattt ccatgcccat ggaagacatt tcaacctacg
      301 aatgaagagg gacacttccc ttttcagtga tgaatttaaa gtagaaacat caaataaagt
      361 acttggttat gatacctctc atatttacac tggacatatt tatggtgaag aaggaagttt
      421 tggccatggg tctgttattg atggaagatt tgaaggattc atccagactc gtggtggcac
      481 attttatgtt gagccagcag agagatatat taaagaccga actctgccat ttcactctgt
      541 catttatcat gaagatgata ttaactatcc ccataaatac ggtcctcagg ggggctgtgc
      601 agatcattca gtatttgaaa gaatgaggaa ataccagatg actggtgtag aggaagtaac
      661 acagatacct caagaagaac atgctgctaa tggtccagaa cttctgagga aaaaacgtac
      721 aacttcagct gaaaaaaata cttgtcagct ttatattcag actgatcatt tgttctttaa
      781 atattacgga acacgagaag ctgtgattgc ccagatatcc agtcatgtta a
//
