LOCUS       sequence-163756-       15472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of human POLQ K121M mutant (a mutation in the 
            conserved ATP-binding site of the Walker A motif in the 
            helicase-like domain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15472)
  AUTHORS   Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, 
            Bylund GO, Doublie S, Johansson E, Ramsden DA, McBride KM, Wood RD
  TITLE     Mechanism of suppression of chromosomal instability by DNA 
            polymerase POLQ.
  JOURNAL   PLoS Genet. 2014 Oct 2;10(10):e1004654. doi: 
            10.1371/journal.pgen.1004654. eCollection 2014 Oct.
  PUBMED    25275444
REFERENCE   2  (bases 1 to 15472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2014-10-2"; volume: "10(10):e1004654. doi"; pages: "
            10.1371/journal.pgen.1004654. eCollection 2014 Oct"
COMMENT     Sequence Label: pCDH-EF1-FHC-POLQ-K121M
FEATURES             Location/Qualifiers
     source          1..15472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1963..2174
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2187..2455
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             10304..10327
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10343..10369
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             10343..10369
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    10380..10952
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10547..10564)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10774..10793
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10961..11560
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10961..10980)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11457..11477
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11567..12155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11620..11640)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12038..12049)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12229..12462
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12534..12655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12571..12590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12625..12644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12674..12809
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12847..12863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12847..12863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12860..12882)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12871..12887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12895..12901,12902..12919,12920..12925))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12940..12961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13078..13095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13249..13837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(14008..14799,14800..14868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14631..14650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14869..14973)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15041..15059
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15097..15119)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     15219..15238
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15432..15454
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15446..15463
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15447..15463
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
     1981 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     2041 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     2101 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     2161 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     2221 ctacctgagg ccgccatcca cgccggttga gtcgcgttct tgcgcctccc gcctgtggtg
     2281 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     2341 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     2401 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     2461 tgtgaccggc gcctactcta gagctagcga attcgaattt aaatcggatc ctagcaccat
     2521 gaatcttctg cgtcggagtg ggaaacggcg gcgttcagaa tcaggctcag attcgttctc
     2581 gggaagcggc ggtgacagca gtgccagccc ccagttcctc tccgggtccg tgctgagccc
     2641 gccgcccggc cttggtcgct gcctgaaggc cgcagctgca ggagaatgca agcctacagt
     2701 tcctgactac gaaatagaca agctactatt ggcaaactgg ggacttccta aagcagttct
     2761 ggaaaaatac cacagttttg gtgtaaaaaa gatgtttgaa tggcaggcag agtgcctttt
     2821 gcttggacaa gtcctggaag gaaagaattt agtttattca gctcctacaa gtgctgggat
     2881 gactcttgtg gcagaattac ttattttgaa gcgggttttg gaaatgcgga agaaagcttt
     2941 gtttattctt ccctttgttt ctgtggctaa agagaagaaa tactacctcc agagtctgtt
     3001 tcaggaagta ggaataaaag tagacggtta tatgggcagc acctctccat caaggcattt
     3061 ctcttcattg gatattgcag tctgcacaat tgagagagcc aatggtctga tcaatcgcct
     3121 catagaggaa aataagatgg atctgttagg aatggtggtt gtggatgaat tacatatgct
     3181 gggagactct caccgagggt atctgctgga acttttgctg accaagattt gctatattac
     3241 tcggaaatca gcatcttgtc aggcagatct agccagttct ctgtctaatg ctgtgcaaat
     3301 cgttggcatg agtgctaccc ttcctaattt ggagcttgtg gcttcctggt tgaatgctga
     3361 actctaccat accgactttc gccctgtacc gcttttggag tcagtaaaag ttggaaattc
     3421 catatatgac tcttcaatga aacttgtgag ggaatttgag cccatgcttc aagtgaaggg
     3481 agatgaggac catgttgtta gcttatgtta tgagacgatt tgtgataacc attcagtatt
     3541 acttttttgt ccatcaaaga aatggtgtga gaagctggca gatatcattg ctcgagagtt
     3601 ttataatcta catcatcaag ctgagggatt ggtgaaaccc tctgaatgcc caccagtaat
     3661 tctggaacaa aaagaactcc tggaagtgat ggatcagtta agacggttgc cttcaggact
     3721 ggactctgta ttacagaaaa ctgtaccatg gggagtagca tttcatcatg caggtcttac
     3781 ttttgaggag agggatatca ttgaaggagc ctttcgtcaa ggtctcattc gagtcttggc
     3841 ggcaacttct actctttctt ctggggtgaa tttacctgca cgtcgtgtga ttattcgaac
     3901 ccctattttt ggtggtcgac ctctagatat tcttacttat aagcagatgg ttggtcgtgc
     3961 tggcaggaaa ggagtggaca cagtaggcga gagtatctta atttgtaaga actctgagaa
     4021 atcaaaaggc atagctctcc ttcagggttc tctaaagcct gttcgcagct gtctgcaaag
     4081 acgagaagga gaagaagtaa ctggcagcat gatacgagct attctggaga taatagttgg
     4141 tggagtggca agtacatcac aagatatgca tacttatgct gcctgcacat ttttggctgc
     4201 aagtatgaaa gaagggaagc aaggaattca gagaaatcaa gagtctgttc agcttggagc
     4261 gattgaggcc tgtgtgatgt ggctactaga aaatgaattc atccagagta cagaagccag
     4321 tgatggaaca gaaggaaagg tgtatcatcc aacacatctt ggttcggcca ctctttcttc
     4381 ttcactttct ccagctgata ctttagatat ttttgctgac ctgcaaagag caatgaaggg
     4441 ctttgtttta gagaatgatc ttcatattct ctatctggtt acacctatgt ttgaggattg
     4501 gactactatt gattggtatc gatttttctg tttatgggag aagttgccaa cttcaatgaa
     4561 aagggtggca gagctagtgg gagttgaaga ggggttcttg gcccgttgtg tgaaaggaaa
     4621 agtagtagcc agaactgaga gacagcatcg acaaatggcc atccataaaa ggtttttcac
     4681 cagtcttgtg ctattagatt taatcagtga agttccctta agggaaataa atcagaaata
     4741 tggatgcaat cgtgggcaga ttcaatcttt gcaacagtca gctgctgttt atgcagggat
     4801 gattacagta ttttccaacc gtctgggctg gcacaacatg gaactactac tttcccaatt
     4861 tcagaagcgt cttacgtttg gcatccagag ggagctgtgt gacctggttc gggtatcctt
     4921 actaaatgct cagagagcca gggttctcta tgcttctggc tttcatactg tggcagacct
     4981 tgctagagca aatattgtgg aggtggaggt gattctgaaa aatgctgtgc ctttcaaaag
     5041 tgcccggaag gcagtggatg aggaagagga agcagttgaa gaacgtcgca atatgcgaac
     5101 tatctgggtg actggcagaa aaggtttaac tgaaagggaa gcagcagccc ttatagtgga
     5161 agaagccaga atgattctgc agcaggactt agttgaaatg ggagtgcaat ggaatccatg
     5221 tgccctgtta cattctagta catgctcatt gactcatagt gagtccgaag taaaggaaca
     5281 cacatttata tcccaaacta agagttctta taaaaaatta acatcaaaga acaaaagtaa
     5341 cacaatattt agtgattctt atattaagca ttcaccaaat atagtgcaag acttaaataa
     5401 aagtagagag catacaagtt cctttaattg taatttccag aatgggaatc aagaacatca
     5461 gagatgttcc attttcagag caagaaaacg ggcctcttta gatataaata aagagaagcc
     5521 aggagcctct cagaatgagg ggaaaacaag tgataagaaa gttgttcaga ctttttcaca
     5581 gaaaacaaaa aaggcacctt tgaatttcaa ttcagaaaag atgagcagaa gttttcgatc
     5641 ttggaaacgt agaaagcatc taaagcgatc tagggacagc agccccctga aagactctgg
     5701 agcgtgtaga atccatttac aaggacagac tctgtctaat cctagtcttt gtgaagaccc
     5761 gtttacctta gatgagaaga aaacggaatt tagaaattca gggccatttg ctaaaaatgt
     5821 atctttgagt ggtaaggaaa aagataataa aacatcattc ccattacaaa taaagcaaaa
     5881 ttgttcatgg aacataacac taactaatga taattttgtg gagcatattg tcacaggatc
     5941 tcagagtaaa aatgtgactt gtcaggccac tagtgtggtt agtgaaaagg gcagaggagt
     6001 agctgttgag gcagaaaaaa taaatgaagt gctgatacaa aatggttcaa aaaaccagaa
     6061 tgtttatatg aaacaccatg acatccatcc aattaaccag tacctgcgaa agcaatctca
     6121 tgaacagaca agcactatta ccaaacagaa aaatataata gagagacaaa tgccctgtga
     6181 agcagtcagt agttacataa atagagactc aaatgttact atcaattgtg aaaggataaa
     6241 gcttaataca gaggaaaata aaccaagtca ttttcaggca ttaggagatg atataagcag
     6301 aactgtgata cccagtgaag tacttccatc agctggagca tttagcaaat cagaaggcca
     6361 gcatgagaat tttctaaata tttctagact acaagaaaaa acaggtactt atacaacaaa
     6421 caaaactaaa aataatcatg tttctgactt aggtttagtc ctctgtgatt ttgaagatag
     6481 tttctatctg gatactcagt cagagaaaat aatacaacag atggcaactg aaaatgccaa
     6541 actaggagca aaggacacca acctggcagc agggataatg cagaagagct tagtccaaca
     6601 gaactcaatg aactcttttc agaaggagtg tcacattcct tttcctgctg aacagcaccc
     6661 tctaggagcg actaagatag atcatttgga ccttaagact gtaggtacta tgaaacaaag
     6721 cagtgattca catggggttg atatcctgac tccagaaagc ccgattttcc attctccaat
     6781 actattggag gaaaatggtc tttttttaaa aaagaatgaa gtttctgtta ctgattcaca
     6841 attaaatagt tttcttcaag gttatcaaac acaagaaact gtgaaaccag ttatacttct
     6901 gattcctcaa aagagaactc ccactggtgt agaaggagaa tgtcttccag ttcctgaaac
     6961 aagtttgaat atgagtgata gtttactatt tgatagcttc agtgatgact atctagtaaa
     7021 agaacaatta cctgatatgc aaatgaaaga accccttcct tcagaagtaa catcaaacca
     7081 ttttagtgat tctctgtgtc tacaagaaga cctaattaaa aaatcaaatg taaatgagaa
     7141 tcaagatacc caccagcagt tgacttgttc caatgatgaa tctattatat tttcagaaat
     7201 ggattctgtt cagatggttg aagctttgga caatgtggat atatttcctg tccaagagaa
     7261 gaatcatact gtagtatctc ctagagcatt agaactaagt gatccagtac ttgatgagca
     7321 ccaccaaggt gatcaagatg gaggagatca agatgaaagg gctgaaaaat caaaattaac
     7381 tgggaccagg caaaatcatt cattcatttg gtcaggggca tcatttgatc taagtccagg
     7441 actgcaaagg attttagata aagtatccag tcctctagaa aatgaaaagc taaaatcaat
     7501 gactataaac ttttccagtt tgaatagaaa aaatacagag ttaaatgaag aacaagaagt
     7561 tatttcaaac ttggagacaa aacaagtgca gggaatttca ttttcttcta ataatgaagt
     7621 aaaaagcaag attgagatgc tagaaaacaa tgccaatcat gatgaaacct catccctctt
     7681 acctcgtaaa gaaagtaata tagttgatga taatggtctc attcctccta cacccattcc
     7741 aacatctgct tctaagctga catttccagg gattcttgaa acacctgtaa acccttggaa
     7801 aactaataat gttttacaac ctggtgaaag ttatttattt ggctcacctt cagatattaa
     7861 aaaccacgat ttaagtccag ggagtagaaa tgggttcaaa gacaacagcc ctattagtga
     7921 cacaagcttt tcacttcagt tatcacagga tggattacag ttaactccag cctcaagcag
     7981 ttcagaaagt ttgtccataa ttgatgtagc aagtgaccaa aatcttttcc aaacattcat
     8041 taaggagtgg cggtgcaaaa agcgattttc catctcactg gcttgtgaaa agattagaag
     8101 tttgacatct tctaaaactg ctactattgg cagtaggttt aagcaagcta gctcacctca
     8161 ggaaattcct attagagatg atggatttcc cattaaaggt tgtgatgaca ccttggtggt
     8221 tggactggca gtatgctggg gtggaaggga tgcctattat ttttcactgc agaaggaaca
     8281 aaagcattct gaaattagtg ccagtttggt tccaccttct ttagatccaa gcctgacttt
     8341 gaaagacagg atgtggtacc ttcaatcttg cttgcgaaag gaatctgata aagaatgttc
     8401 tgttgtcatc tatgacttca tccagagcta taaaattctt cttctttctt gtggcatctc
     8461 cttggagcaa agttatgaag atcctaaggt ggcatgctgg ttactagatc cagattctca
     8521 ggagccgact cttcatagca tagttaccag ttttcttcct catgagcttc cactcctaga
     8581 agggatggag accagccaag ggattcaaag cctggggcta aatgctggca gtgagcattc
     8641 tgggcgatac agagcatctg tggagtccat tctcatcttc aactctatga atcagctcaa
     8701 ctctttgttg cagaaggaaa accttcaaga tgttttccgt aaggtggaaa tgccctctca
     8761 gtactgcttg gccttgctag aactaaatgg aattggcttt agtactgcag aatgtgaaag
     8821 tcagaaacat ataatgcaag ccaagctgga tgcaattgag acccaggcct atcaactagc
     8881 tggccacagt ttttctttca ccagttcaga tgacatcgct gaggttttat ttttggaatt
     8941 gaagttgccc ccaaatagag agatgaaaaa ccaaggcagc aagaaaactc tgggttctac
     9001 cagaagaggg attgacaatg gacgcaagct aaggctggga agacagttca gcactagtaa
     9061 ggacgtttta aataaattaa aggcattaca tcctttacca ggcttgatat tagaatggag
     9121 aagaatcact aatgctatta ccaaagtggt ctttcccctt cagcgggaaa agtgtcttaa
     9181 tccttttctt ggaatggaaa gaatctatcc tgtatcacag tcgcacactg ctacaggacg
     9241 aataaccttt acagaaccaa atattcagaa tgtgccaaga gattttgaaa tcaaaatgcc
     9301 aacactagta ggagaaagcc caccttctca agctgtaggc aaaggcctac ttcccatggg
     9361 cagaggaaaa tataagaagg gtttcagcgt gaatcctaga tgccaggcac agatggagga
     9421 gagagctgca gacagaggaa tgccattttc aattagcatg cgacatgcct ttgtgccttt
     9481 cccaggtggt tcaatactgg ctgctgacta ctctcagctt gaactgagga tcttggctca
     9541 tttatcccat gatcgtcgtc tcattcaagt gttaaacact ggagctgatg ttttcaggag
     9601 cattgcagca gagtggaaga tgattgagcc agagtctgtt ggggatgatc tgaggcagca
     9661 ggcaaaacag atttgctatg ggatcattta tggaatggga gctaaatctt tgggagagca
     9721 gatgggcatt aaagaaaatg atgctgcatg ctatattgac tccttcaaat ccagatacac
     9781 agggattaat caattcatga cagagacagt gaagaattgt aaaagagacg gatttgttca
     9841 gaccattttg ggaaggcgta gatatttgcc aggaatcaaa gacaacaacc cttatcgtaa
     9901 agctcacgct gagcgtcaag ctatcaacac aatagtccaa ggatcagcag ctgatattgt
     9961 caaaatagcc acagttaaca ttcagaagca attagagacc ttccactcaa ccttcaaatc
    10021 ccatggtcat cgagagggta tgctccaaag tgaccgaaca ggattgtcac gaaagagaaa
    10081 actgcaaggg atgttctgcc caatcagagg aggcttcttc atccttcaac tccatgatga
    10141 actcctatat gaagtggcag aagaagatgt tgttcaggta gctcagattg tcaagaatga
    10201 aatggaaagt gctgtaaaac tgtctgtgaa attgaaagtg aaagtgaaaa taggcgccag
    10261 ctggggagag ctaaaggact ttgatgtggc ggccgctgga ggagactaca aggacgacga
    10321 tgacaagtcg gccgctggag gataccccta cgacgtgccc gactacgcct agggccgcgc
    10381 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
    10441 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
    10501 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
    10561 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
    10621 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
    10681 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
    10741 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
    10801 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
    10861 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
    10921 gacgtggttt tcctttgaaa aacacgataa tacctccgga atgaccgagt acaagcccac
    10981 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
    11041 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
    11101 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
    11161 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
    11221 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
    11281 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
    11341 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
    11401 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
    11461 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
    11521 accgcgcacc tggtgcatga cccgcaagcc cggtgcctag gtcgacaatc aacctctgga
    11581 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
    11641 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
    11701 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
    11761 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
    11821 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
    11881 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    11941 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
    12001 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
    12061 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
    12121 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
    12181 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
    12241 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
    12301 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    12361 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    12421 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
    12481 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
    12541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    12661 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    12721 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    12781 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
    12841 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    12901 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    12961 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    13021 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    13081 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    13141 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    13201 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    13261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    13321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    13381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    13441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    13501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    13561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    13621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    13681 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    13741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    13801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    13861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    13921 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    13981 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    14041 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    14101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    14161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    14221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    14281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    14341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    14401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    14461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    14521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    14581 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    14641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    14701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    14761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    14821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    14881 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    14941 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    15001 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    15061 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    15121 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    15181 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    15241 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    15301 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    15361 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    15421 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
