LOCUS       sequence-163759-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA targeting BPTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, 
            Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic
            B, Dumur CI, Landry JW
  TITLE     BPTF inhibits NK cell activity and the abundance of natural 
            cytotoxicity receptor co-ligands.
  JOURNAL   Oncotarget. 2017 May 12;8(38):64344-64357. doi: 
            10.18632/oncotarget.17834. eCollection 2017 Sep 8.
  PUBMED    28969075
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.17834"; journalName: "Oncotarget"; date: 
            "2017-05-12- 12"; volume: "8"; issue: "38"; pages: "64344-64357"
COMMENT     Sequence Label: pLVTHM-Bptf-sh5
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..633
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(34..53)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     530..550
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
       61 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
      121 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
      181 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
      241 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
      301 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
      361 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
      421 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
      481 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
      541 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
      601 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
      661 accgaaagga gcgcacgacc ccatgcatcg gcgtctcgag atatcagtgg tccaggctct
      721 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agataaaata
      781 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
      841 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatac
//
