LOCUS       sequence-163766-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA targeting BPTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, 
            Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic
            B, Dumur CI, Landry JW
  TITLE     BPTF inhibits NK cell activity and the abundance of natural 
            cytotoxicity receptor co-ligands.
  JOURNAL   Oncotarget. 2017 May 12;8(38):64344-64357. doi: 
            10.18632/oncotarget.17834. eCollection 2017 Sep 8.
  PUBMED    28969075
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.17834"; journalName: "Oncotarget"; date: 
            "2017-05-12- 12"; volume: "8"; issue: "38"; pages: "64344-64357"
COMMENT     Sequence Label: pLVTHM-Bptf-sh28
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(129..162)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             182..362
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(387..405)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(388..405)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(695..714)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(928..947)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tctttggatt tgggaatctt ataagttctg tatgagacca cgcgtcccca gcacagagaa
       61 gaccatgatt caagagatca tggtcttctc tgtgcttttt ttacgcgtat cgataccgtc
      121 gcatgggaat aacttcgtat agcatacatt atacgaagtt atgctgcttt ttgcttgtac
      181 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
      241 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
      301 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
      361 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
      421 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
      481 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
      541 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
      601 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
      661 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
      721 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
      781 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
      841 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
      901 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      961 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     1021 cgcttacaga ca
//
