LOCUS       sequence-163774-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty AAV-RAM plasmid w. multiple cloning site (MCS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    55..643
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(108..128)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(526..537)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(676..693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    682..793
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgctagcgg cgcgccagcg atccaccgga tctagataac tgatccttat cgataatcaa
       61 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
      121 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
      181 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
      241 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
      301 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
      361 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
      421 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
      481 gttgccacct ggattctgcg cgggacgccc ttctgctacg tcccttcggc cctcaatcca
      541 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
      601 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacta
      661 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
      781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
      841 aggacagcaa gggggaggat tgggaagaca atagcagact agtacgtctc tcaaggataa
      901 gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc
      961 attacatctg
//
