LOCUS       sequence-163775-         484 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty AAV-RAM plasmid w. multiple cloning site (MCS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..484
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(105..419)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(108..132)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    215..233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    250..268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    286..304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    322..340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    357..375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    393..411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataaggat cagttatcta
       61 gatccggtgg atcgctggcg cgccgctagc gtttaaacac cggtggcgat ctgacggttc
      121 actaaacgag ctctgcttat ataggcctcc caccgtacac gcctacctcg acatacgttc
      181 tctatcactg atagggagta aactcgacat acgttctcta tcactgatag ggataaactc
      241 gacatacgtt ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata
      301 gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcga catcgttctc
      361 tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg agtaaactcg
      421 agacgcgcgt gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
      481 ctcg
//
