LOCUS       sequence-163779-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN designed to target the human AAVS1/PPP1R12C locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D
  TITLE     An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome 
            Editing in Human Pluripotent Stem Cells.
  JOURNAL   Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018.
  PUBMED    24931489
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018."
COMMENT     Sequence Label: AAVS1-TALEN-R
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..155
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             162..182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg gccaccatgg actataagga ccacgacgga gactacaagg
      121 atcatgatat tgattacaaa gacgatgacg ataagatggc cccaaagaag aagcggaagg
      181 tcggtatcca cggagtccca gcagccgtag atttgagaac tttgggatat tcacagcagc
      241 agcaggaaaa gatcaagccc aaagtgaggt cgacagtcgc gcagcatcac gaagcgctgg
      301 tgggtcatgg gtttacacat gcccacatcg tagccttgtc gcagcaccct gcagcccttg
      361 gcacggtcgc cgtcaagtac caggacatga ttgcggcgtt gccggaagcc acacatgagg
      421 cgatcgtcgg tgtggggaaa cagtggagcg gagcccgagc gcttgaggcc ctgttgacgg
      481 tcgcgggaga gctgagaggg cctccccttc agctggacac gggccagttg ctgaagatcg
      541 cgaagcgggg aggagtcacg gcggtcgagg cggtgcacgc gtggcgcaat gcgctcacgg
      601 gagcacccct caacctgacc ccagagcagg tcgtggcaat tgcgagcaac ggagggggaa
      661 agcaggcact cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg cacggactta
      721 cgccagagca ggtcgtggca attgcgagca acggaggggg aaagcaggca ctcgaaaccg
      781 tccagaggtt gctgcctgtg ctgtgccaag cgcacggact aaccccagag caggtcgtgg
      841 caattgcgag caacggaggg ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg
      901 tgctgtgcca agcgcacggg ttgaccccag agcaggtcgt ggcaattgcg agccatgacg
      961 ggg
//
