LOCUS       sequence-163784-        4128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing WIPI1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WIPI1_WT
FEATURES             Location/Qualifiers
     source          1..4128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2007..2106)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2123..2142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2124..2142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2147..2163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2147..2163)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2594..3385
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3478..4066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3967..3986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gaggccgagg ccgcggacgc tcccccgggc ggggttgagt cggcgctcag
      721 ctgcttctct ttcaaccagg actgcacatc cctagcaatt ggaactaaag ccgggtataa
      781 gctgttttct ctgagttctg tggagcagct ggatcaagtc cacggaagca atgaaatccc
      841 ggacgtctac atcgtggagc gcctcttctc cagcagcctg gtggtggtag tcagtcacac
      901 aaaaccacgg cagatgaacg tgtatcactt caagaaaggc acagagatct gtaattacag
      961 ctactccagc aacatcttgt ccataaggct gaaccggcaa aggctgctgg tttgcctaga
     1021 agagtccatt tatattcaca acattaaaga catgaagctg ttgaagaccc tcctggatat
     1081 tcctgcaaac ccaacaggtc tatgtgctct ctctatcaac cattccaatt cttacctggc
     1141 ctatcctgga agcctgactt caggggagat tgtgctttat gatggaaact ccctgaaaac
     1201 agtctgcact attgctgccc atgagggaac actagctgcc atcaccttca atgcctcagg
     1261 ctccaaacta gcaagtgcgt ctgaaaaagg cacagtcatc cgggtgttct ctgtccctga
     1321 tgggcaaaag ctctatgagt tccggagagg gatgaaaagg tatgtgacaa tcagctctct
     1381 agtgttcagt atggattcac aattcctctg cgcctccagt aacaccgaga cggtacacat
     1441 cttcaagctg gaacaggtca ccaacagtcg accagaagag ccttcgacct ggagtggcta
     1501 catgggaaag atgtttatgg ctgctaccaa ctacctccct acccaggtgt cagacatgat
     1561 gcatcaggac agggcttttg ccactgcacg cttgaacttc tccggacaga ggaacatctg
     1621 taccctctca acgatccaga agttgccacg gctgctagtt gcgtcatcca gtggacacct
     1681 ttatatgtac aatttggatc ctcaggatgg aggagagtgt gtcttaatca aaacccacag
     1741 cttgcttggc tcaggaacaa cagaagagaa taaagaaaat gacctcagac cttccttacc
     1801 tcagtcttat gcagcgaccg tagccagacc aagtgcatct tcagcctcca cggtgccagg
     1861 ttattctgag gacggcgggg cgctgcgagg agaagttatt cctgaacatg agtttgcgac
     1921 gggaccagtg tgtcttgatg atgagaatga gtttcctcct ataatcttgt gccgtggaaa
     1981 tcagaagggc aaaacgaagc agtcatgccc aactttcttg tacaaagttg gcattataag
     2041 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2101 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2161 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2221 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     2281 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     2341 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     2401 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2461 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     2521 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     2581 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     2641 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     2701 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     2761 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     2821 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     2881 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     2941 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     3001 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     3061 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     3121 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     3181 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     3241 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     3301 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     3361 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     3421 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3481 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3541 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3601 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3661 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3721 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3781 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3841 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3901 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3961 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4021 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4081 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
