LOCUS       sequence-163806-        4797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TCF4 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TCF4_WT
FEATURES             Location/Qualifiers
     source          1..4797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2197..2296)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2313..2332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2314..2332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2337..2353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2337..2353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2784..3575
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3668..4256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4157..4176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4410..4427
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4586..4613)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4705..4791)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcatcac caacagcgaa tggctgcctt agggacggac aaagagctga
      241 gtgatttact ggatttcagt gcgatgtttt cacctcctgt gagcagtggg aaaaatggac
      301 caacttcttt ggcaagtgga cattttactg gctcaaatgt agaagacaga agtagctcag
      361 ggtcctgggg gaatggagga catccaagcc cgtccaggaa ctatggagat gggactccct
      421 atgaccacat gaccagcagg gaccttgggt cacatgacaa tctctctcca ccttttgtca
      481 attccagaat acaaagtaaa acagaaaggg gctcatactc atcttatggg agagaatcaa
      541 acttacaggg ttgccaccag cagagtctcc ttggaggtga catggatatg ggcaacccag
      601 gaaccctttc gcccaccaaa cctggttccc agtactatca gtattctagc aataatcccc
      661 gaaggaggcc tcttcacagt agtgccatgg aggtacagac aaagaaagtt cgaaaagttc
      721 ctccaggttt gccatcttca gtctatgctc catcagcaag cactgccgac tacaataggg
      781 actcgccagg ctatccttcc tccaaaccag caaccagcac tttccctagc tccttcttca
      841 tgcaagatgg ccatcacagc agtgaccctt ggagctcctc cagtgggatg aatcagcctg
      901 gctatgcagg aatgttgggc aactcttctc atattccaca gtccagcagc tactgtagcc
      961 tgcatccaca tgaacgtttg agctatccat cacactcctc agcagacatc aattccagtc
     1021 ttcctccgat gtccactttc catcgtagtg gtacaaacca ttacagcacc tcttcctgta
     1081 cgcctcctgc caacgggaca gacagtataa tggcaaatag aggaagcggg gcagccggca
     1141 gctcccagac tggagatgct ctggggaaag cacttgcttc gatctattct ccagatcaca
     1201 ctaacaacag cttttcatca aacccttcaa ctcctgttgg ctctcctcca tctctctcag
     1261 caggcacagc tgtttggtct agaaatggag gacaggcctc atcgtctcct aattatgaag
     1321 gacccttaca ctctttgcaa agccgaattg aagatcgttt agaaagactg gatgatgcta
     1381 ttcatgttct ccggaaccat gcagtgggcc catccacagc tatgcctggt ggtcatgggg
     1441 acatgcatgg aatcattgga ccttctcata atggagccat gggtggtctg ggctcagggt
     1501 atggaaccgg ccttctttca gccaacagac attcactcat ggtggggacc catcgtgaag
     1561 atggcgtggc cctgagaggc agccattctc ttctgccaaa ccaggttccg gttccacagc
     1621 ttcctgtcca gtctgcgact tcccctgacc tgaacccacc ccaggaccct tacagaggca
     1681 tgccaccagg actacagggg cagagtgtct cctctggcag ctctgagatc aaatccgatg
     1741 acgagggtga tgagaacctg caagacacga aatcttcgga ggacaagaaa ttagatgacg
     1801 acaagaagga tatcaaatca attactagca ataatgacga tgaggacctg acaccagagc
     1861 agaaggcaga gcgtgagaag gagcggagga tggccaacaa tgcccgagag cgtctgcggg
     1921 tccgtgacat caacgaggct ttcaaagagc tcggccgcat ggtgcagctc cacctcaaga
     1981 gtgacaagcc ccagaccaag ctcctgatcc tccaccaggc ggtggccgtc atcctcagtc
     2041 tggagcagca agtccgagaa aggaatctga atccgaaagc tgcgtgtctg aaaagaaggg
     2101 aggaagagaa ggtgtcctcg gagcctcccc ctctctcctt ggccggccca caccctggaa
     2161 tgggagacgc atcgaatcac atgggacaga tgtaattgcc aactttcttg tacaaagttg
     2221 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     2281 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     2341 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2401 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     2461 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     2521 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     2581 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2641 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2701 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2761 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     2821 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2881 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     2941 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3001 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3061 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3121 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3181 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3241 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3301 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3361 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3421 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3481 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     3541 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     3601 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3661 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3721 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3781 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3841 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3901 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3961 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4021 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4081 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4141 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4201 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4261 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4321 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4381 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4441 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4501 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     4561 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4621 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4681 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4741 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcct
//
