LOCUS       sequence-163816-        4287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing XIAP , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_XIAP_WT
FEATURES             Location/Qualifiers
     source          1..4287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1687..1786)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1803..1822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1804..1822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1827..1843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1827..1843)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2274..3065
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3158..3746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3647..3666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3900..3917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4076..4103)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4195..4281)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgactttt aacagttttg aaggatctaa aacttgtgta cctgcagaca
      241 tcaataagga agaagaattt gtagaagagt ttaatagatt aaaaactttt gctaattttc
      301 caagtggtag tcctgtttca gcatcaacac tggcacgagc agggtttctt tatactggtg
      361 aaggagatac cgtgcggtgc tttagttgtc atgcagctgt agataggtgg caatatggag
      421 actcagcagt tggaagacac aggaaagtat ccccaaattg cagatttatc aacggctttt
      481 atcttgaaaa tagtgccacg cagtctacaa attctggtat ccagaatggt cagtacaaag
      541 ttgaaaacta tctgggaagc agagatcatt ttgccttaga caggccatct gagacacatg
      601 cagactatct tttgagaact gggcaggttg tagatatatc agacaccata tacccgagga
      661 accctgccat gtatagtgaa gaagctagat taaagtcctt tcagaactgg ccagactatg
      721 ctcacctaac cccaagagag ttagcaagtg ctggactcta ctacacaggt attggtgacc
      781 aagtgcagtg cttttgttgt ggtggaaaac tgaaaaattg ggaaccttgt gatcgtgcct
      841 ggtcagaaca caggcgacac tttcctaatt gcttctttgt tttgggccgg aatcttaata
      901 ttcgaagtga atctgatgct gtgagttctg ataggaattt cccaaattca acaaatcttc
      961 caagaaatcc atccatggca gattatgaag cacggatctt tacttttggg acatggatat
     1021 actcagttaa caaggagcag cttgcaagag ctggatttta tgctttaggt gaaggtgata
     1081 aagtaaagtg ctttcactgt ggaggagggc taactgattg gaagcccagt gaagaccctt
     1141 gggaacaaca tgctaaatgg tatccagggt gcaaatatct gttagaacag aagggacaag
     1201 aatatataaa caatattcat ttaactcatt cacttgagga gtgtctggta agaactactg
     1261 agaaaacacc atcactaact agaagaattg atgataccat cttccaaaat cctatggtac
     1321 aagaagctat acgaatgggg ttcagtttca aggacattaa gaaaataatg gaggaaaaaa
     1381 ttcagatatc tgggagcaac tataaatcac ttgaggttct ggttgcagat ctagtgaatg
     1441 ctcagaaaga cagtatgcaa gatgagtcaa gtcagacttc attacagaaa gagattagta
     1501 ctgaagagca gctaaggcgc ctgcaagagg agaagctttg caaaatctgt atggatagaa
     1561 atattgctat cgtttttgtt ccttgtggac atctagtcac ttgtaaacaa tgtgctgaag
     1621 cagttgacaa gtgtcccatg tgctacacag tcattacttt caagcaaaaa atttttatgt
     1681 cttaattgcc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     1741 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     1801 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     1861 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     1921 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     1981 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2041 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2101 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2161 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2221 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2281 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2341 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2401 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2461 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2521 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2581 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2641 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2701 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2761 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2821 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     2881 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     2941 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3001 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3061 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3301 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3721 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3781 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3841 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3901 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3961 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4021 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4081 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4141 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4201 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4261 ctgagccttt cgttttattt gatgcct
//
