LOCUS       sequence-163819-        4299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BMPR1B , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BMPR1B_p.Q203D
FEATURES             Location/Qualifiers
     source          1..4299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1699..1798)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1815..1834)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1816..1834)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1839..1855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1839..1855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2286..3077
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3170..3758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3659..3678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3912..3929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4088..4115)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4207..4293)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcttttg cgaagtgcag gaaaattaaa tgtgggcacc aagaaagagg
      241 atggtgagag tacagccccc accccccgtc caaaggtctt gcgttgtaaa tgccaccacc
      301 attgtccaga agactcagtc aacaatattt gcagcacaga cggatattgt ttcacgatga
      361 tagaagagga tgactctggg ttgcctgtgg tcacttctgg ttgcctagga ctagaaggct
      421 cagattttca gtgtcgggac actcccattc ctcatcaaag aagatcaatt gaatgctgca
      481 cagaaaggaa cgaatgtaat aaagacctac accctacact gcctccattg aaaaacagag
      541 attttgttga tggacctata caccacaggg ctttacttat atctgtgact gtctgtagtt
      601 tgctcttggt ccttatcata ttattttgtt acttccggta taaaagacaa gaaaccagac
      661 ctcgatacag cattgggtta gaacaggatg aaacttacat tcctcctgga gaatccctga
      721 gagacttaat tgagcagtct cagagctcag gaagtggatc aggcctccct ctgctggtcc
      781 aaaggactat agctaaggac attcagatgg tgaaacagat tggaaaaggt cgctatgggg
      841 aagtttggat gggaaagtgg cgtggcgaaa aggtagctgt gaaagtgttc ttcaccacag
      901 aggaagccag ctggttcaga gagacagaaa tatatcagac agtgttgatg aggcatgaaa
      961 acattttggg tttcattgct gcagatatca aagggacagg gtcctggacc cagttgtacc
     1021 taatcacaga ctatcatgaa aatggttccc tttatgatta tctgaagtcc accaccctag
     1081 acgctaaatc aatgctgaag ttagcctact cttctgtcag tggcttatgt catttacaca
     1141 cagaaatctt tagtactcaa ggcaaaccag caattgccca tcgagatctg aaaagtaaaa
     1201 acattctggt gaagaaaaat ggaacttgct gtattgctga cctgggcctg gctgttaaat
     1261 ttattagtga tacaaatgaa gttgacatac cacctaacac tcgagttggc accaaacgct
     1321 atatgcctcc agaagtgttg gacgagagct tgaacagaaa tcacttccag tcttacatca
     1381 tggctgacat gtatagtttt ggcctcatcc tttgggaggt tgctaggaga tgtgtatcag
     1441 gaggtatagt ggaagaatac cagcttcctt atcatgacct agtgcccagt gacccctctt
     1501 atgaggacat gagggagatt gtgtgcatca agaagttacg cccctcattc ccaaaccggt
     1561 ggagcagtga tgagtgtcta aggcagatgg gaaaactcat gacagaatgc tgggctcaca
     1621 atcctgcatc aaggctgaca gccctgcggg ttaagaaaac acttgccaaa atgtcagagt
     1681 cccaggacat taaactctgc ccaactttct tgtacaaagt tggcattata agaaagcatt
     1741 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     1801 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1861 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1921 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1981 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2041 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2101 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2161 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2221 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2281 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2341 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2401 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2461 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2521 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2581 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2641 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2701 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2761 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2821 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2881 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2941 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3001 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3061 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     3121 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3301 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3901 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3961 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4021 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4081 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4141 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4201 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4261 ccagtcttcc gactgagcct ttcgttttat ttgatgcct
//
