LOCUS       sequence-163820-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty pAAV-CRAM plasmid w. multiple cloning site (MCS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(7..40)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(91..124)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    207..240
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    291..324
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    369..957
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(422..442)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(840..851)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(990..1007)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tccggaataa cttcgtatag gatactttat acgaagttat gcagaatggt agctggattg
       61 tagctgctat tagcaatatg aaacctctta ataacttcgt atagcataca ttatacgaag
      121 ttatggcgcg cccgccatat gccgttaatt aagcgccatg gcggcaattg ccgaccggtg
      181 cggagctccg gactagtcga gctagcataa cttcgtataa agtatcctat acgaagttat
      241 ttgccttaac ccagaaatta tcactgttat tctttagaat ggtgcaaaga ataacttcgt
      301 ataatgtatg ctatacgaag ttatggcgcc agcgatccac cggatctaga taactgatcc
      361 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
      421 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
      481 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
      541 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
      601 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
      661 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
      721 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
      781 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gcccttctgc tacgtccctt
      841 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
      901 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
      961 gataccgtcg actagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     1021 tg
//
