LOCUS       sequence-163823-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-CRAM-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(10..43)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(94..127)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(426..445)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     695..713
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gactccggaa taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga
       61 ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg
      121 aagttatggc gcgccgtcga cttacttgta cagctcgtcc atgccgtaca ggaacaggtg
      181 gtggcggccc tcggagcgct cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt
      241 gatgtccagc ttggtgtcca cgtagtagta gccgggcagt tgcacgggct tcttggccat
      301 gtagatggtc ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca gggcctggtg
      361 gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg cctcccagcc
      421 catggtcttc ttctgcatta cggggccgtc gggggggaag ttggtgccgc gcatcttcac
      481 cttgtagatc agcgtgccgt cctgcaggga ggagtcctgg gtcacggtca ccagaccgcc
      541 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcttgta
      601 atcggggatg tcggcggggt gcttcacgta cgccttggag ccgtacatga actgggggga
      661 caggatgtcc caggcgaagg gcagggggcc gcccttggtc accttcagct tggcggtctg
      721 ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcat
      781 ggagccctcc atgcgcacct tgaagcgcat gaactctttg atgacggcca tgttgttgtc
      841 ctcggaggag gcggtgccgg agctgccgct gccggtgctg ccgatgccat gccccaggaa
      901 caggtggtgg cggccctcgg agcgctcgta ctgttccacg atggtgtagt cctcgttgtg
      961 ggaggtgatg tccagc
//
