LOCUS       sequence-163824-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-CRAM-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(79..112)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(163..196)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      206..215
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(345..363)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     613..632
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     807..830
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattatcgat aaggatcagt tatctagatc
       61 cggtggatcg ctggcgccat aacttcgtat agcatacatt atacgaagtt attctttgca
      121 ccattctaaa gaataacagt gataatttct gggttaaggc aaataacttc gtataggata
      181 ctttatacga agttatgcta gcggcgccac catggtgagc aagggcgagg aggtcatcaa
      241 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
      301 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      361 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
      421 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
      481 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
      541 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
      601 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
      661 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
      721 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
      781 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
      841 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctg
//
