LOCUS       sequence-163836-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of an extended membrane scaffold protein 
            (MSP1E3D1) for Nanodisc formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Denisov IG, Baas BJ, Grinkova YV, Sligar SG
  TITLE     Cooperativity in cytochrome P450 3A4: linkages in substrate binding,
            spin state, uncoupling, and product formation.
  JOURNAL   J Biol Chem. 2007 Mar 9. 282(10):7066-76.
  PUBMED    17213193
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Mar 9. 282(10):7066-76."
COMMENT     Sequence Label: pMSP1E3D1
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggtcat catcatcatc
       61 atcatcacga ttatgatatt cctactactg agaatttgta ttttcagggt tctaccttca
      121 gtaaacttcg cgaacaactg ggccccgtga cgcaggaatt ctgggacaac ctggaaaaag
      181 aaaccgaggg actgcgtcag gaaatgtcca aagatttaga agaggtgaag gccaaggttc
      241 agccatatct cgatgacttt cagaaaaaat ggcaggaaga gatggaatta tatcgtcaaa
      301 aggtggaacc gctgcgtgcg gaactgcaag agggggcacg ccaaaaactc catgagctcc
      361 aagagaagct cagcccatta ggcgaagaaa tgcgcgatcg cgcccgtgca catgttgatg
      421 cactccggac tcatttggcg ccatatctcg atgactttca gaaaaaatgg caggaagaga
      481 tggaattata tcgtcaaaag gtggaaccgc tgcgtgcgga actgcaagag ggggcacgcc
      541 aaaaactcca tgagctccaa gagaagctca gcccattagg cgaagaaatg cgcgatcgcg
      601 cccgtgcaca tgttgatgca ctccggactc atttggcgcc gtattcggat gaacttcgcc
      661 agcgtttggc cgcacgtctc gaggcgctga aagaaaacgg gggtgcccgc ttggctgagt
      721 accacgcgaa agcgacagaa cacctgagca ccttgagcga aaaagcgaaa ccggcgctgg
      781 aagatctacg ccagggctta ttgcctgttc ttgagagctt taaagtcagt tttctgtcag
      841 ctctggaaga atatactaaa aagctgaata cccagtaagc
//
