LOCUS       sequence-163838-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin 
            JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM
  TITLE     Modification of superoxide dismutase 1 (SOD1) properties by a GFP 
            tag--implications for research into amyotrophic lateral sclerosis 
            (ALS).
  JOURNAL   PLoS One. 2010 . 5(3):e9541.
  PUBMED    20221404
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010 . 5(3):e9541."
COMMENT     Sequence Label: pF151 pcDNA3.1(+)SOD1WT
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(652..669)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    658..865
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcgagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga
      121 tccactagtc cagtgtggtg gaattccatg gcgacgaagg ccgtgtgcgt gctgaagggc
      181 gacggcccag tgcagggcat catcaatttc gagcagaagg aaagtaatgg accagtgaag
      241 gtgtggggaa gcattaaagg actgactgaa ggcctgcatg gattccatgt tcatgagttt
      301 ggagataata cagcaggctg taccagtgca ggtcctcact ttaatcctct atccagaaaa
      361 cacggtgggc caaaggatga agagaggcat gttggagact tgggcaatgt gactgctgac
      421 aaagatggtg tggccgatgt gtctattgaa gattctgtga tctcactctc aggagaccat
      481 tgcatcattg gccgcacact ggtggtccat gaaaaagcag atgacttggg caaaggtgga
      541 aatgaagaaa gtacaaagac aggaaacgct ggaagtcgtt tggcttgtgg tgtaattggg
      601 atcgcccaat aacgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
      661 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      721 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
      781 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
      841 aagacaatag caggcatgct ggggatgcgg tgggctctat g
//
