LOCUS       sequence-163847-        4800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NBPF15 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NBPF15_WT
FEATURES             Location/Qualifiers
     source          1..4800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2679..2778)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2795..2814)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2796..2814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2819..2835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2819..2835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3266..4057
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4150..4738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4639..4658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gtggtatcag ccggcccttt gtccagcgag aaggcagaga tgaacattct
      721 agaaatcaat gagaaattgc gcccccagtt ggcagagaag aaacagcagt tcagaaacct
      781 caaagagaaa tgttttctaa ctcaactggc cggcttcctg gccaaccgac agaagaaata
      841 caaatatgaa gagtgcaaag atctcataaa atttatgctg aggaatgagc gacagttcaa
      901 ggaggagaag cttgcagagc agctcaagca agctgaggag ctcaggcaat ataaagtcct
      961 ggttcacgct caggaacgag agctgaccca gttaagggag aagttacggg aagggagaga
     1021 tgcctcccgc tcattgaatg agcatctcca ggccctcctc actccggatg agccggacaa
     1081 gtcccagggg caggacctcc aagaacagct ggctgagggg tgtagactga cacagcacct
     1141 tgtccaaaag ctcagcccag aaaatgacaa cgatgacgat gaagatgttc aagttgaggt
     1201 ggctgagaaa gtgcagaaat cgtctgcccc cagggagatg cagaaggctg aagaaaagga
     1261 agtccctgag gactcactgg aggaatgtgc catcacttgt tcaaatagcc atggccctta
     1321 tgactccaac cagccacata agaaaaccaa aatcacattt gaggaagaca aagtcgactc
     1381 aactctcatt ggctcatcct ctcatgttga atgggaggat gctgtacaca ttattccaga
     1441 aaatgaaagt gatgatgagg aagaggaaga aaaagggcca gtgtctccca ggaatctgca
     1501 ggagtctgaa gaggaggaag tcccccagga gtcctgggat gaaggttatt cgactctctc
     1561 aattcctcct gaaatgttgg cctcgtacca gtcttacagc agcacatttc actcattaga
     1621 ggaacagcaa gtctgcatgg ctgttgacat aggcagacat cggtgggatc aagtgaaaaa
     1681 ggaggaccaa gaggcaacag gtcccaggct cagcagggag ctgctggatg agaaagagcc
     1741 tgaagtcttg caggactcac tggatagatg ttattcaact ccttcaggtt gtcttgaact
     1801 gactgactca tgccagccct acagaagtgc cttttacgta ttggagcaac agcgtgttgg
     1861 cttggctatt gacatggatg aaattgaaaa gtaccaagaa gtggaagaag accaagaccc
     1921 atcatgcccc aggctcagca gggagctgct ggatgagaaa gagcctgaag tcttgcagga
     1981 ctcactggat agatgttatt caactccttc agattatctt gaactgcctg acttaggcca
     2041 gccctacagc agtgctgttt actcattgga ggaacagtac cttggcttgg ctcttgccgt
     2101 ggacagaatt aaaaaggacc aagaagagga agaagaccaa ggcccaccat gccccaggct
     2161 cagcagggag ctgctggagg tagtagagcc tgaagtcttg caggactcac tggatagatg
     2221 ttattcaact ccttccagtt gtcttgaaca gcctgactcc tgccagccct atggaagttc
     2281 cttttatgca ttggaggaaa aacatgttgg cttttctctt gacgtgggag aaattgaaaa
     2341 gaaggggaag gggaagaaaa gaaggggaag aagatcaaag aagaaaagaa gaaggggaag
     2401 aaaagaaggg gaagatgaca acccaccatg ccccaggctc tacggcgtgc tgatggaagt
     2461 ggaagagcct gaagtcttac aggactcact ggatagatgt tattcgactc cgtcaatgta
     2521 ctttgaacaa cctgactcat tccagcacta cagaagtgtg ttttactcat ttgaggaaga
     2581 gcatatcagc ttcgcccttt acgtggacaa taggtttttt actttgacgg tgacaagtct
     2641 ccacctggtg ttccagatgg gagtcatatt cccacaatac ccaactttct tgtacaaagt
     2701 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2761 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2821 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2881 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     2941 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     3001 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     3061 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     3121 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     3181 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     3241 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     3301 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     3361 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     3421 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3481 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3541 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3601 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3661 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3721 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3781 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     3841 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     3901 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     3961 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     4021 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     4081 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4141 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4201 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4261 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4321 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4381 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4441 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4501 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4561 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4621 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4681 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4741 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
