LOCUS       sequence-163887-        4662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KEAP1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KEAP1_WT
FEATURES             Location/Qualifiers
     source          1..4662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2541..2640)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2657..2676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2658..2676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2681..2697)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2681..2697)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3128..3919
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4012..4600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4501..4520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaag
      661 agttggcatg cagccagatc ccaggcctag cggggctggg gcctgctgcc gattcctgcc
      721 cctgcagtca cagtgccctg agggggcagg ggacgcggtg atgtacgcct ccactgagtg
      781 caaggcggag gtgacgccct cccagcatgg caaccgcacc ttcagctaca ccctggagga
      841 tcataccaag caggcctttg gcatcatgaa cgagctgcgg ctcagccagc agctgtgtga
      901 cgtcacactg caggtcaagt accaggatgc accggccgcc cagttcatgg cccacaaggt
      961 ggtgctggcc tcatccagcc ctgtcttcaa ggccatgttc accaacgggc tgcgggagca
     1021 gggcatggag gtggtgtcca ttgagggtat ccaccccaag gtcatggagc gcctcattga
     1081 attcgcctac acggcctcca tctccatggg cgagaagtgt gtcctccacg tcatgaacgg
     1141 tgctgtcatg taccagatcg acagcgttgt ccgtgcctgc agtgacttcc tggtgcagca
     1201 gctggacccc agcaatgcca tcggcatcgc caacttcgct gagcagattg gctgtgtgga
     1261 gttgcaccag cgtgcccggg agtacatcta catgcatttt ggggaggtgg ccaagcaaga
     1321 ggagttcttc aacctgtccc actgccaact ggtgaccctc atcagccggg acgacctgaa
     1381 cgtgcgctgc gagtccgagg tcttccacgc ctgcatcaac tgggtcaagt acgactgcga
     1441 acagcgacgg ttctacgtcc aggcgctgct gcgggccgtg cgctgccact cgttgacgcc
     1501 gaacttcctg cagatgcagc tgcagaagtg cgagatcctg cagtccgact cccgctgcaa
     1561 ggactacctg gtcaagatct tcgaggagct caccctgcac aagcccacgc aggtgatgcc
     1621 ctgccgggcg cccaaggtgg gccgcctgat ctacaccgcg ggcggctact tccgacagtc
     1681 gctcagctac ctggaggctt acaaccccag tgacggcacc tggctccggt tggcggacct
     1741 gcaggtgccg cggagcggcc tggccggctg cgtggtgggc gggctgttgt acgccgtggg
     1801 cggcaggaac aactcgcccg acggcaacac cgactccagc gccctggact gttacaaccc
     1861 catgaccaat cagtggtcgc cctgcgcccc catgagcgtg ccccgtaacc gcatcggggt
     1921 gggggtcatc gatggccaca tctatgccgt cggcggctcc cacggctgca tccaccacaa
     1981 cagtgtggag aggtatgagc cagagcggga tgagtggcac ttggtggccc caatgctgac
     2041 acgaaggatc ggggtgggcg tggctgtcct caatcgtctc ctttatgccg tggggggctt
     2101 tgacgggaca aaccgcctta attcagctga gtgttactac ccagagagga acgagtggcg
     2161 aatgatcaca gcaatgaaca ccatccgaag cggggcaggc gtctgcgtcc tgcacaactg
     2221 tatctatgct gctgggggct atgatggtca ggaccagctg aacagcgtgg agcgctacga
     2281 tgtggaaaca gagacgtgga ctttcgtagc ccccatgaag caccggcgaa gtgccctggg
     2341 gatcactgtc caccagggga gaatctacgt ccttggaggc tatgatggtc acacgttcct
     2401 ggacagtgtg gagtgttacg acccagatac agacacctgg agcgaggtga cccgaatgac
     2461 atcgggccgg agtggggtgg gcgtggctgt caccatggag ccctgccgga agcagattga
     2521 ccagcagaac tgtaccagtt gcccaacttt cttgtacaaa gttggcatta taagaaagca
     2581 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2641 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2701 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2761 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2821 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2881 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2941 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     3001 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     3061 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     3121 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     3181 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     3241 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3301 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3361 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3421 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3481 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3541 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3601 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3661 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3721 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3781 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3841 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3901 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3961 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4021 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4081 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4141 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4201 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4261 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4321 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4381 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4441 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4501 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4561 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4621 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
