LOCUS       sequence-16389-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Fan M, Rhee J, St-Pierre J, Handschin C, Puigserver P, Lin J, Jaeger
            S, Erdjument-Bromage H, Tempst P, Spiegelman BM
  TITLE     Suppression of mitochondrial respiration through recruitment of p160
            myb binding protein to PGC-1alpha: modulation by p38 MAPK.
  JOURNAL   Genes Dev 2004 Feb 1;18(3):278-89. Epub 2004 Jan 26.
  PUBMED    14744933
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev";
            date: "2004-02-1"; volume: "18(3)"; pages: "278-89. Epub 2004 Jan 
            26"
COMMENT     Sequence Label: pcDNA-p160(1-200)
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             610..630
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             684..713
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             729..746
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(769..786)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    775..886
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcggagatga agagccctac gaaagctgag cctgcgactc ccgcagaagc ggcgcaaagc
       61 gaccgccaca gcctgctgga gcacagccgc gagttcttgg acttcttctg ggacattgcg
      121 aaaccggatc aggaaacgcg gctccgggcc acggagaagt tgttggagta cttgcgcaca
      181 aggcccaatg attcggagat gaaatatgcc ctgaagcgcc taatcactgg gcttggggtg
      241 ggccgagaag ccgctaggcc ctgctacagc ctggcgctgg cacagctgtt gcagtctttt
      301 gaagacatcc cattgtgtga catcctggat cagatacaag aaaaatacag cctacaagcc
      361 atgaacaagg caatgatgag acctagtctc tttgcaaacc tttttggagt gctagccctc
      421 tttcagtcag gccgcctagt gaaggacaaa gaggccctga tgaagtccgt gcaattgctg
      481 aagatcctgt cccaacaccc caaccactta cagggacagc caataaaggc tctggtggac
      541 atcctctctg aggtcccaga gtccatgttc caggagatcc tgccaaaggt cctcaagggt
      601 aacatggatc cgaaaaagaa gcgtaaggta ggcggttgag atatccagca cagtggcggc
      661 cgctcgagtc tagagggccc ttcgaacaaa aactcatctc agaagaggat ctgaatatgc
      721 ataccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
      781 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      841 gtgccactcc cactgtcctt tccctaataa atgaggaaat tgcatcgcat tgtctgagta
      901 ggtgtcatct atct
//
