LOCUS       sequence-163895-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-RAM-ChR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-ChR2-WPREpA
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..930
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      940..949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(991..1012)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accatggact atggcggcgc tttgtctgcc gtcggacgcg aacttttgtt cgttactaat
       61 cctgtggtgg tgaacgggtc cgtcctggtc cctgaggatc aatgttactg tgccggatgg
      121 attgaatctc gcggcacgaa cggcgctcag accgcgtcaa atgtcctgca gtggcttgca
      181 gcaggattca gcattttgct gctgatgttc tatgcctacc aaacctggaa atctacatgc
      241 ggctgggagg agatctatgt gtgcgccatt gaaatggtta aggtgattct cgagttcttt
      301 tttgagttta agaatccctc tatgctctac cttgccacag gacaccgggt gcagtggctg
      361 cgctatgcag agtggctgct cacttgtcct gtcatcctta tccgcctgag caacctcacc
      421 ggcctgagca acgactacag caggagaacc atgggactcc ttgtctcaga catcgggact
      481 atcgtgtggg gggctaccag cgccatggca accggctatg ttaaagtcat cttcttttgt
      541 cttggattgt gctatggcgc gaacacattt tttcacgccg ccaaagcata tatcgagggt
      601 tatcatactg tgccaaaggg tcggtgccgc caggtcgtga ccggcatggc atggctgttt
      661 ttcgtgagct ggggtatgtt cccaattctc ttcattttgg ggcccgaagg ttttggcgtc
      721 ctgagcgtct atggctccac cgtaggtcac acgattattg atctgatgag taaaaattgt
      781 tgggggttgt tgggacacta cctgcgcgtc ctgatccacg agcacatatt gattcacgga
      841 gatatccgca aaaccaccaa actgaacatc ggcggaacgg agatcgaggt cgagactctc
      901 gtcgaagacg aagccgaggc cggagccgtg ccagcggccg ccaccatggt gagcaagggc
      961 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1021 cacaagttca gcgtg
//
