LOCUS       sequence-163897-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1 , part of the Target 
            Accelerator Plasmid Collection. (Please note this plasmid contains 
            S45A mutation, not T45A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.S33A_S37A_T41A_T45A
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             192..2537
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDAGIHAGATATAPA
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(2539..2638)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2655..2674)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2656..2674)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2679..2695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2679..2695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3126..3917
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4010..4598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4499..4518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4752..4769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4928..4955)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5047..5133)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggctact caagctgatt tgatggagtt ggacatggcc atggaaccag
      241 acagaaaagc ggctgttagt cactggcagc aacagtctta cctggacgct ggaatccatg
      301 ctggtgccac tgccacagct cctgctctga gtggtaaagg caatcctgag gaagaggatg
      361 tggatacctc ccaagtcctg tatgagtggg aacagggatt ttctcagtcc ttcactcaag
      421 aacaagtagc tgatattgat ggacagtatg caatgactcg agctcagagg gtacgagctg
      481 ctatgttccc tgagacatta gatgagggca tgcagatccc atctacacag tttgatgctg
      541 ctcatcccac taatgtccag cgtttggctg aaccatcaca gatgctgaaa catgcagttg
      601 taaacttgat taactatcaa gatgatgcag aacttgccac acgtgcaatc cctgaactga
      661 caaaactgct aaatgacgag gaccaggtgg tggttaataa ggctgcagtt atggtccatc
      721 agctttctaa aaaggaagct tccagacacg ctatcatgcg ttctcctcag atggtgtctg
      781 ctattgtacg taccatgcag aatacaaatg atgtagaaac agctcgttgt accgctggga
      841 ccttgcataa cctttcccat catcgtgagg gcttactggc catctttaag tctggaggca
      901 ttcctgccct ggtgaaaatg cttggttcac cagtggattc tgtgttgttt tatgccatta
      961 caactctcca caacctttta ttacatcaag aaggagctaa aatggcagtg cgtttagctg
     1021 gtgggctgca gaaaatggtt gccttgctca acaaaacaaa tgttaaattc ttggctatta
     1081 cgacagactg ccttcaaatt ttagcttatg gcaaccaaga aagcaagctc atcatactgg
     1141 ctagtggtgg accccaagct ttagtaaata taatgaggac ctatacttac gaaaaactac
     1201 tgtggaccac aagcagagtg ctgaaggtgc tatctgtctg ctctagtaat aagccggcta
     1261 ttgtagaagc tggtggaatg caagctttag gacttcacct gacagatcca agtcaacgtc
     1321 ttgttcagaa ctgtctttgg actctcagga atctttcaga tgctgcaact aaacaggaag
     1381 ggatggaagg tctccttggg actcttgttc agcttctggg ttcagatgat ataaatgtgg
     1441 tcacctgtgc agctggaatt ctttctaacc tcacttgcaa taattataag aacaagatga
     1501 tggtctgcca agtgggtggt atagaggctc ttgtgcgtac tgtccttcgg gctggtgaca
     1561 gggaagacat cactgagcct gccatctgtg ctcttcgtca tctgaccagc cgacaccaag
     1621 aagcagagat ggcccagaat gcagttcgcc ttcactatgg actaccagtt gtggttaagc
     1681 tcttacaccc accatcccac tggcctctga taaaggctac tgttggattg attcgaaatc
     1741 ttgccctttg tcccgcaaat catgcacctt tgcgtgagca gggtgccatt ccacgactag
     1801 ttcagttgct tgttcgtgca catcaggata cccagcgccg tacgtccatg ggtgggacac
     1861 agcagcaatt tgtggagggg gtccgcatgg aagaaatagt tgaaggttgt accggagccc
     1921 ttcacatcct agctcgggat gttcacaacc gaattgttat cagaggacta aataccattc
     1981 cattgtttgt gcagctgctt tattctccca ttgaaaacat ccaaagagta gctgcagggg
     2041 tcctctgtga acttgctcag gacaaggaag ctgcagaagc tattgaagct gagggagcca
     2101 cagctcctct gacagagtta cttcactcta ggaatgaagg tgtggcgaca tatgcagctg
     2161 ctgttttgtt ccgaatgtct gaggacaagc cacaagatta caagaaacgg ctttcagttg
     2221 agctgaccag ctctctcttc agaacagagc caatggcttg gaatgagact gctgatcttg
     2281 gacttgatat tggtgcccag ggagaacccc ttggatatcg ccaggatgat cctagctatc
     2341 gttcttttca ctctggtgga tatggccagg atgccttggg tatggacccc atgatggaac
     2401 atgagatggg tggccaccac cctggtgctg actatccagt tgatgggctg ccagatctgg
     2461 ggcatgccca ggacctcatg gatgggctgc ctccaggtga cagcaatcag ctggcctggt
     2521 ttgatactga cctgtaattg ccaactttct tgtacaaagt tggcattata agaaagcatt
     2581 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     2641 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2701 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2761 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     2821 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2881 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2941 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     3001 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     3061 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     3121 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     3181 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3241 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     3301 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     3361 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     3421 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     3481 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3541 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3601 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3661 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3721 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3781 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3841 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3901 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     3961 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4021 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4081 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4141 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4201 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4261 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4321 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4381 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4441 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4501 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4561 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4621 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4681 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4741 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4801 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4861 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4921 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4981 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     5041 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     5101 ccagtcttcc gactgagcct ttcgttttat ttgatgcct
//
