LOCUS       sequence-16391-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Fan M, Rhee J, St-Pierre J, Handschin C, Puigserver P, Lin J, Jaeger
            S, Erdjument-Bromage H, Tempst P, Spiegelman BM
  TITLE     Suppression of mitochondrial respiration through recruitment of p160
            myb binding protein to PGC-1alpha: modulation by p38 MAPK.
  JOURNAL   Genes Dev 2004 Feb 1;18(3):278-89. Epub 2004 Jan 26.
  PUBMED    14744933
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev";
            date: "2004-02-1"; volume: "18(3)"; pages: "278-89. Epub 2004 Jan 
            26"
COMMENT     Sequence Label: pcDNA-p160(400-580)
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             540..560
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             614..643
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             659..676
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(699..716)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    705..816
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagctatgt ggcctggttg cgggacatgt tcctgcagcc tgacctgaac tccttggttg
       61 acttcagcac tgccaaccag aagagagctc aggacgcctc gttgaatgtg cctgagcgag
      121 ctgtattccg gctccggaag tggatcatcc accgcctggt cagccttgtg gatcatttgc
      181 atctggagaa ggatgaagct gtggttgagc aaatagccag gttttgcttg ttccatgcct
      241 tctttaagac gaagaaggct acgccccaga tcccagagac gaagcagcac ttctccttcc
      301 ctttggacga ccgcaaccgt ggggtctttg tcagtgcctt cttcagccta ctgcagacgc
      361 tcagtgtgaa gttcaggcag acaccagacc tggctgaaaa tgggaagcct tggacttacc
      421 gcctggttca gttggcagac atgctgttga accataaccg caatgtaacc agtgtgacat
      481 ccttgacaac acagcagcgt caggcctggg accagatgat gagtactctg aaggaagatc
      541 cgaaaaagaa gcgtaaggta ggcggttgag atatccagca cagtggcggc cgctcgagtc
      601 tagagggccc ttcgaacaaa aactcatctc agaagaggat ctgaatatgc ataccggtca
      661 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
      721 ccagccatct gttgtttgcc cctcccccgt gccttccttg acccctggag gtgccactcc
      781 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcttct
      841 atc
//
