LOCUS       sequence-163915-        4740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RAF1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RAF1_p.L613V
FEATURES             Location/Qualifiers
     source          1..4740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             129..920
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1013..1601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1502..1521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1755..1772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1931..1958)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2050..2136)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2185..2207
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2199..2216
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2200..2216
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2232..2331
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4282..4381)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4398..4417)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4399..4417)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4422..4438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4422..4438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
       61 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
      121 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
      181 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
      241 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
      301 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
      361 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
      421 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
      481 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
      541 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
      601 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
      661 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
      721 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
      781 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
      841 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
      901 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
      961 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1021 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1081 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1141 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1201 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1261 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1321 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1381 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1441 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1501 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1561 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1621 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1681 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1741 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1801 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1861 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     1921 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     1981 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     2041 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     2101 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     2161 cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta
     2221 agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
     2281 ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagttggc accatggagc
     2341 acatacaggg agcttggaag acgatcagca atggttttgg attcaaagat gccgtgtttg
     2401 atggctccag ctgcatctct cctacaatag ttcagcagtt tggctatcag cgccgggcat
     2461 cagatgatgg caaactcaca gatccttcta agacaagcaa cactatccgt gttttcttgc
     2521 cgaacaagca aagaacagtg gtcaatgtgc gaaatggaat gagcttgcat gactgcctta
     2581 tgaaagcact caaggtgagg ggcctgcaac cagagtgctg tgcagtgttc agacttctcc
     2641 acgaacacaa aggtaaaaaa gcacgcttag attggaatac tgatgctgcg tctttgattg
     2701 gagaagaact tcaagtagat ttcctggatc atgttcccct cacaacacac aactttgctc
     2761 ggaagacgtt cctgaagctt gccttctgtg acatctgtca gaaattcctg ctcaatggat
     2821 ttcgatgtca gacttgtggc tacaaatttc atgagcactg tagcaccaaa gtacctacta
     2881 tgtgtgtgga ctggagtaac atcagacaac tcttattgtt tccaaattcc actattggtg
     2941 atagtggagt cccagcacta ccttctttga ctatgcgtcg tatgcgagag tctgtttcca
     3001 ggatgcctgt tagttctcag cacagatatt ctacacctca cgccttcacc tttaacacct
     3061 ccagtccctc atctgaaggt tccctctccc agaggcagag gtcgacatcc acacctaatg
     3121 tccacatggt cagcaccacc ctgcctgtgg acagcaggat gattgaggat gcaattcgaa
     3181 gtcacagcga atcagcctca ccttcagccc tgtccagtag ccccaacaat ctgagcccaa
     3241 caggctggtc acagccgaaa acccccgtgc cagcacaaag agagcgggca ccagtatctg
     3301 ggacccagga gaaaaacaaa attaggcctc gtggacagag agattcaagc tattattggg
     3361 aaatagaagc cagtgaagtg atgctgtcca ctcggattgg gtcaggctct tttggaactg
     3421 tttataaggg taaatggcac ggagatgttg cagtaaagat cctaaaggtt gtcgacccaa
     3481 ccccagagca attccaggcc ttcaggaatg aggtggctgt tctgcgcaaa acacggcatg
     3541 tgaacattct gcttttcatg gggtacatga caaaggacaa cctggcaatt gtgacccagt
     3601 ggtgcgaggg cagcagcctc tacaaacacc tgcatgtcca ggagaccaag tttcagatgt
     3661 tccagctaat tgacattgcc cggcagacgg ctcagggaat ggactatttg catgcaaaga
     3721 acatcatcca tagagacatg aaatccaaca atatatttct ccatgaaggc ttaacagtga
     3781 aaattggaga ttttggtttg gcaacagtaa agtcacgctg gagtggttct cagcaggttg
     3841 aacaacctac tggctctgtc ctctggatgg ccccagaggt gatccgaatg caggataaca
     3901 acccattcag tttccagtcg gatgtctact cctatggcat cgtattgtat gaactgatga
     3961 cgggggagct tccttattct cacatcaaca accgagatca gatcatcttc atggtgggcc
     4021 gaggatatgc ctccccagat cttagtaagc tatataagaa ctgccccaaa gcaatgaaga
     4081 ggctggtagc tgactgtgtg aagaaagtaa aggaagagag gcctcttttt ccccagatcc
     4141 tgtcttccat tgagctgctc caacactctg taccgaagat caaccggagc gcttccgagc
     4201 catccttgca tcgggcagcc cacactgagg atatcaatgc ttgcacgctg accacgtccc
     4261 cgaggctgcc tgtcttctag ttgccaactt tcttgtacaa agttggcatt ataagaaagc
     4321 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     4381 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     4441 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     4501 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     4561 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     4621 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     4681 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
//
