LOCUS       sequence-163916-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-RAM-ArchT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-ArchT-WPREpA
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(83..796,797..799))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(86..796,797..799))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(128..149)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     477..496
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     736..757
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tctttcacaa attttgtaat ccagaggttg attatcgata aggatcagtt atctagatcc
       61 ggtggatcgc tggcgcgcct tcttacttgt acagctcgtc catgccgaga gtgatcccgg
      121 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      181 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      241 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      301 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      361 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      421 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      481 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      541 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
      601 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaaa
      661 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
      721 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
      781 gctcctcgcc cttgctcact gctactaccg gtcggggctc gggggcctcg gtgtcgccca
      841 ggatggccct gctcctcagc aggatgaagc cgaagcccac tttggcggtc acgtccagca
      901 ccatgaacag cagggtctcg attcccagtc ccaccactcc agctccctcg gttccgatga
      961 tccacaggat ggggtaggcg gtccacagca ccagcaccag ggcggtcagg gtgttgaagg
     1021 tgctagccac ctctggtccc ctctcctta
//
