LOCUS       sequence-163918-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tetracycline-inducible SH-tagged (streptavidin-binding 
            peptide and HA tag) version of Mst1. Used in conjunction with Flp-In
            cells to generate single copy transgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Rawat SJ, Creasy CL, Peterson JR, Chernoff J
  TITLE     The tumor suppressor Mst1 promotes changes in the cellular redox 
            state by phosphorylation and inactivation of peroxiredoxin-1 
            protein.
  JOURNAL   J Biol Chem. 2013 Mar 22;288(12):8762-71. doi: 
            10.1074/jbc.M112.414524. Epub 2013 Feb 5.
  PUBMED    23386615
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.414524"; journalName: "J Biol Chem"; date: 
            "2013-03-22- 22"; volume: "288"; issue: "12"; pages: "8762-71"
COMMENT     Sequence Label: pcDNA5/FRT/TO/SH/GW-Mst1
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             254..280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             311..334
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             371..394
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    407..431
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtaccg
      181 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
      241 gctcgagacc atgtacccat acgatgttcc cgactacgcc ggtaccgagc tcggatccac
      301 catggctagc tggagccacc cgcagttcga gaaaggtgga ggttccggag gtggatcggg
      361 aggtggatcg tggagccacc cgcagttcga aaaagcggcc gatatcacaa gtttgtacaa
      421 aaaagcaggc ttcatggaga cggtacagct gaggaacccg ccgcgccggc agctgaaaaa
      481 gttggatgaa gatagtttaa ccaaacaacc agaagaagta tttgatgtct tagagaaact
      541 tggagaaggg tcctatggca gcgtatacaa agctattcat aaagagaccg gccagattgt
      601 tgctattaag caagttcctg tggaatcaga cctccaggag ataatcaaag aaatctctat
      661 aatgcagcaa tgtgacagcc ctcatgtagt caaatattat ggcagttatt ttaagaacac
      721 agacttatgg atcgttatgg agtactgtgg ggctggttct gtatctgata tcattcgatt
      781 acgaaataaa acgttaacag aagatgaaat agctacaata ttaca
//
