LOCUS       sequence-163921-        1936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tetracycline-inducible SH-tagged (streptavidin-binding 
            peptide and HA tag) version of Mst1. Used in conjunction with Flp-In
            cells to generate single copy transgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Rawat SJ, Creasy CL, Peterson JR, Chernoff J
  TITLE     The tumor suppressor Mst1 promotes changes in the cellular redox 
            state by phosphorylation and inactivation of peroxiredoxin-1 
            protein.
  JOURNAL   J Biol Chem. 2013 Mar 22;288(12):8762-71. doi: 
            10.1074/jbc.M112.414524. Epub 2013 Feb 5.
  PUBMED    23386615
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.414524"; journalName: "J Biol Chem"; date: 
            "2013-03-22- 22"; volume: "288"; issue: "12"; pages: "8762-71"
COMMENT     Sequence Label: pcDNA5/FRT/TO/SH/GW-Mst1
FEATURES             Location/Qualifiers
     source          1..1936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             254..280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             311..334
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             371..394
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    407..431
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1899..1923)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtaccg
      181 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
      241 gctcgagacc atgtacccat acgatgttcc cgactacgcc ggtaccgagc tcggatccac
      301 catggctagc tggagccacc cgcagttcga gaaaggtgga ggttccggag gtggatcggg
      361 aggtggatcg tggagccacc cgcagttcga aaaagcggcc gatatcacaa gtttgtacaa
      421 aaaagcaggc ttcatggaga cggtacagct gaggaacccg ccgcgccggc agctgaaaaa
      481 gttggatgaa gatagtttaa ccaaacaacc agaagaagta tttgatgtct tagagaaact
      541 tggagaaggg tcctatggca gcgtatacaa agctattcat aaagagaccg gccagattgt
      601 tgctattaag caagttcctg tggaatcaga cctccaggag ataatcaaag aaatctctat
      661 aatgcagcaa tgtgacagcc ctcatgtagt caaatattat ggcagttatt ttaagaacac
      721 agacttatgg atcgttatgg agtactgtgg ggctggttct gtatctgata tcattcgatt
      781 acgaaataaa acgttaacag aagatgaaat agctacaata ttacaatcaa ctcttaaggg
      841 acttgaatac cttcatttta tgagaaaaat acaccgagat atcaaggcag gaaatatttt
      901 gctaaataca gaaggacatg caaaacttgc agattttggg gtagcaggtc aacttacaga
      961 taccatggcc aagcggaata cagtgatagg aacaccattt tggatggctc cagaagtgat
     1021 tcaggaaatt ggatacaact gtgtagcaga catctggtcc ctgggaataa ctgccataga
     1081 aatggctgaa ggaaagcccc cttatgctga tatccatcca atgagggcaa tcttcatgat
     1141 tcctacaaat cctcctccca cattccgaaa accagagcta tggtcagata actttacaga
     1201 ttttgtgaaa cagtgtcttg taaagagccc tgagcagagg gccacagcca ctcagctcct
     1261 gcagcaccca tttgtcagga gtgccaaagg agtgtcaata ctgcgagact taattaatga
     1321 agccatggat gtgaaactga aacgccagga atcccagcag cgggaagtgg accaggacga
     1381 tgaagaaaac tcagaagagg atgaaatgga ttctggcacg atggttcgag cagtgggtga
     1441 tgagatgggc actgtccgag tagccagcac catgactgat ggagccaata ctatgattga
     1501 gcacgatgac acgttgccat cacaactggg caccatggtg atcaatgcag aggatgagga
     1561 agaggaagga actatgaaaa gaagggatga gaccatgcag cctgcgaaac catcctttct
     1621 tgaatatttt gaacaaaaag aaaaggaaaa ccagatcaac agctttggca agagtgtacc
     1681 tggtccactg aaaaattctt cagattggaa aataccacag gatggagact acgagtttct
     1741 taagagttgg acagtggagg accttcagaa gaggctcttg gccctggacc ccatgatgga
     1801 gcaggagatt gaagagatcc ggcagaagta ccagtccaag cggcagccca tcctggatgc
     1861 catagaggct aagaagagac ggcaacaaaa cttctgagac ccagctttct tgtacaaagt
     1921 ggtgacgtaa gctagg
//
