LOCUS       sequence-163922-        4932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STAT1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STAT1_p.Y701F
FEATURES             Location/Qualifiers
     source          1..4932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     136..153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(312..339)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(431..517)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     566..588
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     580..597
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     581..597
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    613..712
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2855..2954)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2971..2990)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2972..2990)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2995..3011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2995..3011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3442..4233
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4326..4914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4815..4834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
       61 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      241 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
      301 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
      361 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
      421 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
      481 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
      541 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
      601 aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
      661 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagttgg caccatgtct
      721 cagtggtacg aacttcagca gcttgactca aaattcctgg agcaggttca ccagctttat
      781 gatgacagtt ttcccatgga aatcagacag tacctggcac agtggttaga aaagcaagac
      841 tgggagcacg ctgccaatga tgtttcattt gccaccatcc gttttcatga cctcctgtca
      901 cagctggatg atcaatatag tcgcttttct ttggagaata acttcttgct acagcataac
      961 ataaggaaaa gcaagcgtaa tcttcaggat aattttcagg aagacccaat ccagatgtct
     1021 atgatcattt acagctgtct gaaggaagaa aggaaaattc tggaaaacgc ccagagattt
     1081 aatcaggctc agtcggggaa tattcagagc acagtgatgt tagacaaaca gaaagagctt
     1141 gacagtaaag tcagaaatgt gaaggacaag gttatgtgta tagagcatga aatcaagagc
     1201 ctggaagatt tacaagatga atatgacttc aaatgcaaaa ccttgcagaa cagagaacac
     1261 gagaccaatg gtgtggcaaa gagtgatcag aaacaagaac agctgttact caagaagatg
     1321 tatttaatgc ttgacaataa gagaaaggaa gtagttcaca aaataataga gttgctgaat
     1381 gtcactgaac ttacccagaa tgccctgatt aatgatgaac tagtggagtg gaagcggaga
     1441 cagcagagcg cctgtattgg ggggccgccc aatgcttgct tggatcagct gcagaactgg
     1501 ttcactatag ttgcggagag tctgcagcaa gttcggcagc agcttaaaaa gttggaggaa
     1561 ttggaacaga aatacaccta cgaacatgac cctatcacaa aaaacaaaca agtgttatgg
     1621 gaccgcacct tcagtctttt ccagcagctc attcagagct cgtttgtggt ggaaagacag
     1681 ccctgcatgc caacgcaccc tcagaggccg ctggtcttga agacaggggt ccagttcact
     1741 gtgaagttga gactgttggt gaaattgcaa gagctgaatt ataatttgaa agtcaaagtc
     1801 ttatttgata aagatgtgaa tgagagaaat acagtaaaag gatttaggaa gttcaacatt
     1861 ttgggcacgc acacaaaagt gatgaacatg gaggagtcca ccaatggcag tctggcggct
     1921 gaatttcggc acctgcaatt gaaagaacag aaaaatgctg gcaccagaac gaatgagggt
     1981 cctctcatcg ttactgaaga gcttcactcc cttagttttg aaacccaatt gtgccagcct
     2041 ggtttggtaa ttgacctcga gacgacctct ctgcccgttg tggtgatctc caacgtcagc
     2101 cagctcccga gcggttgggc ctccatcctt tggtacaaca tgctggtggc ggaacccagg
     2161 aatctgtcct tcttcctgac tccaccatgt gcacgatggg ctcagctttc agaagtgctg
     2221 agttggcagt tttcttctgt caccaaaaga ggtctcaatg tggaccagct gaacatgttg
     2281 ggagagaagc ttcttggtcc taacgccagc cccgatggtc tcattccgtg gacgaggttt
     2341 tgtaaggaaa atataaatga taaaaatttt cccttctggc tttggattga aagcatccta
     2401 gaactcatta aaaaacacct gctccctctc tggaatgatg ggtgcatcat gggcttcatc
     2461 agcaaggagc gagagcgtgc cctgttgaag gaccagcagc cggggacctt cctgctgcgg
     2521 ttcagtgaga gctcccggga aggggccatc acattcacat gggtggagcg gtcccagaac
     2581 ggaggcgaac ctgacttcca tgcggttgaa ccctacacga agaaagaact ttctgctgtt
     2641 actttccctg acatcattcg caattacaaa gtcatggctg ctgagaatat tcctgagaat
     2701 cccctgaagt atctgtatcc aaatattgac aaagaccatg cctttggaaa gtattactcc
     2761 aggccaaagg aagcaccaga gccaatggaa cttgatggcc ctaaaggaac tggatttatc
     2821 aagactgagt tgatttctgt gtctgaagtg taattgccaa ctttcttgta caaagttggc
     2881 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     2941 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     3001 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     3061 aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg
     3121 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
     3181 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     3241 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     3301 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     3361 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     3421 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     3481 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     3541 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     3601 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     3661 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     3721 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     3781 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     3841 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     3901 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     3961 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     4021 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     4081 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     4141 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     4201 aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc
     4261 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4321 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4381 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4441 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4501 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4561 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4621 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4681 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4741 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4801 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4861 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4921 agcaacgcgg cc
//
