LOCUS       sequence-163923-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-RAM-NLS-mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             22..717
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     primer_bind     complement(823..843)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cccaagaaga agaggaaggt ggtgagcgag ctgattaagg agaacatgca catgaagctg
       61 tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag
      121 ccctacgagg gcacccagac catgagaatc aaggcggtcg agggcggccc tctccccttc
      181 gccttcgaca tcctggctac cagcttcatg tacggcagca aaaccttcat caaccacacc
      241 cagggcatcc ccgacttctt taagcagtcc ttccccgagg gcttcacatg ggagagagtc
      301 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
      361 tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg ccctgtgatg
      421 cagaagaaaa cactcggctg ggaggcctcc accgagaccc tgtaccccgc tgacggcggc
      481 ctggaaggca gagccgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac
      541 ttgaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcgtctac
      601 tatgtggaca gaagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag
      661 cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca cagatgagtc
      721 gacggcgcgc cagcgatcca ccggatctag ataactgatc cttatcgata atcaacctct
      781 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
      841 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
      901 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
      961 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     1021 tgccaccacc tgtcagctcc tttc
//
