LOCUS       sequence-163926-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-RAM-NLS-mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..786)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     CDS             complement(787..807)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(828..852)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(849..887)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    899..917
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    935..953
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    970..988
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1006..1024
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataaggat cagttatcta
       61 gatccggtgg atcgctggcg cgccgtcgac tcatctgtgc cccagtttgc tagggaggtc
      121 gcagtatctg gccacagcca cctcgtgctg ctcgacgtag gtctctttgt cggcctcctt
      181 gattctttcc agtcttctgt ccacatagta gacgccgggc atcttgaggt tcttagcggg
      241 tttcttggat ctgtatgtgg tcttcaagtt gcagatcagg tggcccccgc ccacgagctt
      301 cagggccatg tcggctctgc cttccaggcc gccgtcagcg gggtacaggg tctcggtgga
      361 ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatggga agttcacccc
      421 tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt
      481 cagcacgccc ccgtcttcgt atgtggtgac tctctcccat gtgaagccct cggggaagga
      541 ctgcttaaag aagtcgggga tgccctgggt gtggttgatg aaggttttgc tgccgtacat
      601 gaagctggta gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccgccttgat
      661 tctcatggtc tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaagtg
      721 gtggttgttc acggtgccct ccatgtacag cttcatgtgc atgttctcct taatcagctc
      781 gctcaccacc ttcctcttct tcttgggggc cattaaacac cggtggcgat ctgacggttc
      841 actaaacgag ctctgcttat ataggcctcc caccgtacac gcctacctcg acatacgttc
      901 tctatcactg atagggagta aactcgacat acgttctcta tcactgatag ggataaactc
      961 gacatacgtt ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata
     1021 gggagtaaa
//
