LOCUS       sequence-163929-        1906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tetracycline-inducible SH-tagged (streptavidin-binding 
            peptide and HA tag) version of Mst2. Used in conjunction with Flp-In
            cells to generate single copy transgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Rawat SJ, Creasy CL, Peterson JR, Chernoff J
  TITLE     The tumor suppressor Mst1 promotes changes in the cellular redox 
            state by phosphorylation and inactivation of peroxiredoxin-1 
            protein.
  JOURNAL   J Biol Chem. 2013 Mar 22;288(12):8762-71. doi: 
            10.1074/jbc.M112.414524. Epub 2013 Feb 5.
  PUBMED    23386615
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.414524"; journalName: "J Biol Chem"; date: 
            "2013-03-22- 22"; volume: "288"; issue: "12"; pages: "8762-71"
COMMENT     Sequence Label: pcDNA5/FRT/TO/SH/GW-Mst2
FEATURES             Location/Qualifiers
     source          1..1906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             254..280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             311..334
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             371..394
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    407..431
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             1084..1092
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtaccg
      181 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
      241 gctcgagacc atgtacccat acgatgttcc cgactacgcc ggtaccgagc tcggatccac
      301 catggctagc tggagccacc cgcagttcga gaaaggtgga ggttccggag gtggatcggg
      361 aggtggatcg tggagccacc cgcagttcga aaaagcggcc gatatcacaa gtttgtacaa
      421 aaaagttggc atggagcagc cgccggcgcc taagagtaaa ctaaaaaagc tgagtgaaga
      481 cagtttgact aagcagcctg aagaagtttt tgatgtatta gagaagcttg gagaagggtc
      541 ttatggaagt gtatttaaag caatacacaa ggaatccggt caagttgtcg caattaaaca
      601 agtacctgtt gaatcagatc ttcaggaaat aatcaaagaa atttccataa tgcagcaatg
      661 tgacagccca tatgttgtaa agtactatgg cagttatttt aagaatacag acctctggat
      721 tgttatggag tactgtggcg ctggctctgt ctcagacata attagattac gaaacaagac
      781 attaatagaa gatgaaattg caaccattct taaatctaca ttgaaaggac tagaatattt
      841 gcactttatg agaaaaatac acagagatat aaaagctgga aatattctcc tcaatacaga
      901 aggacatgca aaattggcag attttggagt ggctggtcag ttaacagata caatggcaaa
      961 acgcaatact gtaataggaa ctccattttg gatggctcct gaggtgattc aagaaatagg
     1021 ctataactgt gtggccgaca tctggtccct tggcattact tctatagaaa tggctgaagg
     1081 aaaacctcct tatgctgata tacatccaat gagggctatt tttatgattc ccacaaatcc
     1141 accaccaaca ttcagaaagc cagaactttg gtccgatgat ttcaccgatt ttgttaaaaa
     1201 gtgtttggtg aagaatcctg agcagagagc tactgcaaca caacttttac agcatccttt
     1261 tatcaagaat gccaaacctg tatcaatatt aagagacctg atcacagaag ctatggagat
     1321 caaagctaaa agacatgagg aacagcaacg agaattggaa gaggaagaag aaaattcgga
     1381 tgaagatgag ctggattccc acaccatggt gaagactagt gtggagagtg tgggcaccat
     1441 gcgggccaca agcacgatga gtgaaggggc ccagaccatg attgaacata atagcacgat
     1501 gttggaatcc gacttgggga ccatggtgat aaacagtgag gatgaggaag aagaagatgg
     1561 aactatgaaa agaaatgcaa cctcaccaca agtacaaaga ccatctttca tggactactt
     1621 tgataagcaa gacttcaaga ataagagtca cgaaaactgt aatcagaaca tgcatgaacc
     1681 cttccctatg tccaaaaacg tttttcctga taactggaaa gttcctcaag atggagactt
     1741 tgactttttg aaaaatctaa gtttagaaga actacagatg cggttaaaag cactggaccc
     1801 catgatggaa cgggagatag aagaacttcg tcagagatac actgcgaaaa gacagcccat
     1861 tctggatgcg atggatgcaa agaaaagaag gcagcaaaac ttttga
//
