LOCUS       sequence-16393-e        1399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAST_19a
FEATURES             Location/Qualifiers
     source          1..1399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(41..58)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(41..57)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(266..285)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     385..407
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(445..463)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gnggcgntct agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaan
       61 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      181 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
      241 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      301 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      361 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
      421 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      481 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      541 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      601 gataaatgct tcaataatga caagagagaa taacatgact gcttggcgca atggtagcgc
      661 gttcgactct agatcgaaag gttgggcgtt cgatccgctc agtggtcaaa ttttttgttt
      721 tttaagtagt aatataatac aatttaattc aaaattacac gcaaaaattt aataaagtag
      781 tccaaaatgc taatcgtgtg aaaaaatgcc ttctggtaat gtatttctca cacgaaaatt
      841 tgttgaaatt ggtttttgac acaagcgaat gttgcantat tttcttgatt tttctacaaa
      901 tttgaaaatc aaatttcacg actgcaagta taacattaaa aaacaatata cccaagccca
      961 caaaaaacgt tattctgaaa aattggtatt gaaaaaggaa nantatgagt attccacatt
     1021 ttccgngncc nccnttattc cctttttngn ggcatttttg ccttcctgnt tttggctccc
     1081 ccnnaaaacg ctggngnaaa ataaaaaang nctgaaaatc anntggggng cccnagnggn
     1141 ttaaattcna actgganntn annagnggta aaatctttgn agtttccccc ccaaaaagtt
     1201 ttccaagaag annnntttna agnttnnctn tgggggngga antttcccgn atnacccngg
     1261 naaaaaaant tngnncccnn anancctttt ccaaagagtt gnggngaaaa ccccccccna
     1321 aanctttnnn nggggnanaa aaaantnggg gnccccacnn nnaaaaccgn cccttnttnn
     1381 ncangggaaa agaancctt
//
