LOCUS       sequence-163936-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the nDHFR-link1-His6-Ub and UBA1Ta in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pND-Ub17-deltaE2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(615..842)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(855..875)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaagaccacg aacttcagac ttgatgaacg caatgggtgg ctgttggctg tggcagtaat
       61 catcaaattc aattgcttta tctaagctga tatcagtgaa gacaacggcc tggaatttag
      121 aaaggtgctc cttggtcaaa tcaccggtta aggcagagac gaggacagca ttgttcagct
      181 cttgtagctt ctgtacacaa gcttgagcac ggttttgccc aacatcattc tccgagagga
      241 agaagttgct tgataagtcc cacagctcca cgttaccatc atcatgcaag gttacagact
      301 tgacacccgc aaggacaagg ttctttgcga tctcagcacc gagaccctgg agtccagaga
      361 cgaggacgtt ggagccgaag aggcgcttca tggtctcgcg cccatagacg gcgagctggc
      421 ggctgtgcag gtcctcgtcg atctcgttcc ccctccctgc catggctgcg tctccttcct
      481 ccctagtgac ctccccctcc ccggcgcggg tcttcttctg caagtcctcg acctcgccgg
      541 cgacgatctc ccgcttccgg ggaagcatag ctgtttcctg tctctagacc ggcgcgcccc
      601 cccgaactag ttcacccacc tctgagacgg agtaccaggt gcaaggtgga ctctttctgg
      661 atgttgtagt cagacagggt acgaccatct tccagctgtt ttccggcaaa gatcaacctc
      721 tgctgatcag gaggaatgcc ttccttatct tggatctttg ccttgacatt ctcgatggtg
      781 tcactgggct ccacctcgag ggtgatggtc ttaccagtca gagtcttcac gaagatctgc
      841 atggatccca tggcgccctg aaaatacagg ttttcggtcg ttg
//
