LOCUS       sequence-163945-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the nDHFR-link1-His6-Ub and UBC4 Sc in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pND-Ub20-deltaE1
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             72..83
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             528..545
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             567..587
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             600..827
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 atagagattg acatccctat cagtgataga gatactgagc acatcagcag gacgcactga
       61 ccgaattcat taaagaggag aaaggtacca tggttcgacc attgaactgc atcgtcgccg
      121 tgtcccaaaa tatggggatt ggcaagaacg gagacctacc ctggcctccg ctcaggaacg
      181 agttcaagta cttccaaaga atgaccacaa cctcttcagt ggaaggtaaa cagaatctgg
      241 tgattatggg taggaaaacc tggttctcca ttcctgagaa gaatcgacct ttaaaggaca
      301 gaattaatat agttctcagt agagaactca aagaaccacc acgaggagct cattttcttg
      361 ccaaaagttt ggatgatgcc ttaagactta ttgaacaacc ggaattggca ttaattaagg
      421 cggctcagcg ggctcgggaa gcggagcggg atctggcagc ggcggtagcg caggcagcag
      481 cgggtcaagc ggtgcctcga gcggcgcggc cgcaaatgtc gtactaccat caccatcacc
      541 atcacgatta cgatatccca acgaccgaaa acctgtattt tcagggcgcc atgggatcca
      601 tgcagatctt cgtgaagact ctgactggta agaccatcac cctcgaggtg gagcccagtg
      661 acaccatcga gaatgtcaag gcaaagatcc aagataagga aggcattcct cctgatcagc
      721 agaggttgat ctttgccgga aaacagctgg aagatggtcg taccctgtct gactacaaca
      781 tccagaaaga gtccaccttg cacctggtac tccgtctcag aggtgggtga gaattctaag
      841 gaaatccatt atgtcttctt ctaaacgtat tgctaaagaa ctaagtgatc tagaaagaga
      901 tccacctact tcatgttcag ccggtccagt cggcgatgat ctatatcact ggcaagcatc
      961 catcatggga cctgccgatt ccccatatgc cggcgg
//
