LOCUS       sequence-163946-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the nDHFR-link1-His6-Ub and UBC4 Sc in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pND-Ub20-deltaE1
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(549..776)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(789..809)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(831..848)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaccgacaca agtgggccgg cgacgatctc ccgcttccgg ggaagcatag ctgtttcctg
       61 tcggaattgt ggcgcgcctt atacagcgta tttctttgtc cattctctgg ctgtagcttc
      121 gtacttgggt ctgtcagtct tgtagatatg agcgatttct ggtactaaag gatcgtcagg
      181 attagcgtct gttaacaaag aacagatgga taataggacc ttcgatagag ttagagctgg
      241 agaccattga tcctttagga tgtccagaca gatgttacca ttggcattga tatttggatg
      301 atatatcttg gttgtgaagg agatctttgg tggcttgaat gggtagtcgg ttgggaaatg
      361 gatagacaag aagaaaacac cgccggcata tggggaatcg gcaggtccca tgatggatgc
      421 ttgccagtga tatagatcat cgccgactgg accggctgaa catgaagtag gtggatctct
      481 ttctagatca cttagttctt tagcaatacg tttagaagaa gacataatgg atttccttag
      541 aattctcacc cacctctgag acggagtacc aggtgcaagg tggactcttt ctggatgttg
      601 tagtcagaca gggtacgacc atcttccagc tgttttccgg caaagatcaa cctctgctga
      661 tcaggaggaa tgccttcctt atcttggatc tttgccttga cattctcgat ggtgtcactg
      721 ggctccacct cgagggtgat ggtcttacca gtcagagtct tcacgaagat ctgcatggat
      781 cccatggcgc cctgaaaata caggttttcg gtcgttggga tatcgtaatc gtgatggtga
      841 tggtgatggt agtacgacat t
//
