LOCUS       sequence-163951-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the nDHFR-link1-His6-Ub, UBCH5C Hs and UBA1Ta in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pND-Ub21-UBCH5C Hs
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             74..85
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             530..547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             569..589
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             602..829
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc aggacgcact
       61 gaccgaattc attaaagagg agaaaggtac catggttcga ccattgaact gcatcgtcgc
      121 cgtgtcccaa aatatgggga ttggcaagaa cggagaccta ccctggcctc cgctcaggaa
      181 cgagttcaag tacttccaaa gaatgaccac aacctcttca gtggaaggta aacagaatct
      241 ggtgattatg ggtaggaaaa cctggttctc cattcctgag aagaatcgac ctttaaagga
      301 cagaattaat atagttctca gtagagaact caaagaacca ccacgaggag ctcattttct
      361 tgccaaaagt ttggatgatg ccttaagact tattgaacaa ccggaattgg cattaattaa
      421 ggcggctcag cgggctcggg aagcggagcg ggatctggca gcggcggtag cgcaggcagc
      481 agcgggtcaa gcggtgcctc gagcggcgcg gccgcaaatg tcgtactacc atcaccatca
      541 ccatcacgat tacgatatcc caacgaccga aaacctgtat tttcagggcg ccatgggatc
      601 catgcagatc ttcgtgaaga ctctgactgg taagaccatc accctcgagg tggagcccag
      661 tgacaccatc gagaatgtca aggcaaagat ccaagataag gaaggcattc ctcctgatca
      721 gcagaggttg atctttgccg gaaaacagct ggaagatggt cgtaccctgt ctgactacaa
      781 catccagaaa gagtccacct tgcacctggt actccgtctc agaggtgggt gagaattcta
      841 aggaaatcca ttatggcgct gaaacggatt aataaggaac ttagtgattt ggcccgtgac
      901 cctccagcac aatgttctgc aggtccagtt ggggatgata tgtttcattg gcaagccaca
      961 attatgggac ctaatgacag cccatatcaa ggcggtgtat tctttttgac aattcatttt
     1021 cctac
//
