LOCUS       sequence-163953-        4926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STAT1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STAT1_WT
FEATURES             Location/Qualifiers
     source          1..4926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     122..144
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     136..153
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     137..153
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    169..268
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2405..2504)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2521..2540)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2522..2540)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2545..2561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2545..2561)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2992..3783
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3876..4464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4365..4384
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4618..4635
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4794..4821)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(join(4913..4926,1..73))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
       61 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
      121 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt
      181 ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata
      241 atgccaactt tgtacaaaaa agttggcatg tctcagtggt acgaacttca gcagcttgac
      301 tcaaaattcc tggagcaggt tcaccagctt tatgatgaca gttttcccat ggaaatcaga
      361 cagtacctgg cacagtggtt agaaaagcaa gactgggagc acgctgccaa tgatgtttca
      421 tttgccacca tccgttttca tgacctcctg tcacagctgg atgatcaata tagtcgcttt
      481 tctttggaga ataacttctt gctacagcat aacataagga aaagcaagcg taatcttcag
      541 gataattttc aggaagaccc aatccagatg tctatgatca tttacagctg tctgaaggaa
      601 gaaaggaaaa ttctggaaaa cgcccagaga tttaatcagg ctcagtcggg gaatattcag
      661 agcacagtga tgttagacaa acagaaagag cttgacagta aagtcagaaa tgtgaaggac
      721 aaggttatgt gtatagagca tgaaatcaag agcctggaag atttacaaga tgaatatgac
      781 ttcaaatgca aaaccttgca gaacagagaa cacgagacca atggtgtggc aaagagtgat
      841 cagaaacaag aacagctgtt actcaagaag atgtatttaa tgcttgacaa taagagaaag
      901 gaagtagttc acaaaataat agagttgctg aatgtcactg aacttaccca gaatgccctg
      961 attaatgatg aactagtgga gtggaagcgg agacagcaga gcgcctgtat tggggggccg
     1021 cccaatgctt gcttggatca gctgcagaac tggttcacta tagttgcgga gagtctgcag
     1081 caagttcggc agcagcttaa aaagttggag gaattggaac agaaatacac ctacgaacat
     1141 gaccctatca caaaaaacaa acaagtgtta tgggaccgca ccttcagtct tttccagcag
     1201 ctcattcaga gctcgtttgt ggtggaaaga cagccctgca tgccaacgca ccctcagagg
     1261 ccgctggtct tgaagacagg ggtccagttc actgtgaagt tgagactgtt ggtgaaattg
     1321 caagagctga attataattt gaaagtcaaa gtcttatttg ataaagatgt gaatgagaga
     1381 aatacagtaa aaggatttag gaagttcaac attttgggca cgcacacaaa agtgatgaac
     1441 atggaggagt ccaccaatgg cagtctggcg gctgaatttc ggcacctgca attgaaagaa
     1501 cagaaaaatg ctggcaccag aacgaatgag ggtcctctca tcgttactga agagcttcac
     1561 tcccttagtt ttgaaaccca attgtgccag cctggtttgg taattgacct cgagacgacc
     1621 tctctgcccg ttgtggtgat ctccaacgtc agccagctcc cgagcggttg ggcctccatc
     1681 ctttggtaca acatgctggt ggcggaaccc aggaatctgt ccttcttcct gactccacca
     1741 tgtgcacgat gggctcagct ttcagaagtg ctgagttggc agttttcttc tgtcaccaaa
     1801 agaggtctca atgtggacca gctgaacatg ttgggagaga agcttcttgg tcctaacgcc
     1861 agccccgatg gtctcattcc gtggacgagg ttttgtaagg aaaatataaa tgataaaaat
     1921 tttcccttct ggctttggat tgaaagcatc ctagaactca ttaaaaaaca cctgctccct
     1981 ctctggaatg atgggtgcat catgggcttc atcagcaagg agcgagagcg tgccctgttg
     2041 aaggaccagc agccggggac cttcctgctg cggttcagtg agagctcccg ggaaggggcc
     2101 atcacattca catgggtgga gcggtcccag aacggaggcg aacctgactt ccatgcggtt
     2161 gaaccctaca cgaagaaaga actttctgct gttactttcc ctgacatcat tcgcaattac
     2221 aaagtcatgg ctgctgagaa tattcctgag aatcccctga agtatctgta tccaaatatt
     2281 gacaaagacc atgcctttgg aaagtattac tccaggccaa aggaagcacc agagccaatg
     2341 gaacttgatg gccctaaagg aactggatat atcaagactg agttgatttc tgtgtctgaa
     2401 gtgtacccaa ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     2461 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     2521 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     2581 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc
     2641 agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg
     2701 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     2761 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     2821 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     2881 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     2941 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa
     3001 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     3061 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     3121 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     3181 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     3241 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     3301 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     3361 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     3421 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     3481 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     3541 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     3601 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     3661 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     3721 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     3781 taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     3841 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3901 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3961 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4021 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4081 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4141 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4201 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4261 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4321 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4381 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4441 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4501 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4561 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4621 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4681 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4741 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     4801 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     4861 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     4921 tactca
//
